黄芪多糖对糖尿病大鼠心肌细胞凋亡的影响  

Effect of Astragalus polysaccharide on myocardial cell apoptosis in diabetic rats

作　　者：于胜男[1] 曹琼丹 鲁美丽[1] 杨育红[1] 

机构地区：[1]辽宁医学院心脑血管药物研究重点实验室,锦州121001

出　　处：《中药药理与临床》2015年第4期102-105,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然基金项目(项目编号81374008)

摘　　要：目的:探讨黄芪多糖对糖尿病大鼠心肌细胞凋亡蛋白及能量代谢的影响。方法:SD大鼠50只,随机分为对照组、糖尿病心肌病组、黄芪多糖200、400、800mg/kg组,后4组腹腔注射链脲佐菌素55 mg/kg制备糖尿病模型。造模后第7周起,对照组和糖尿病心肌病组给予纯净水,其余三组分别给予黄芪多糖200,400,800mg/kg连续给药6周,于第12周末用超声心动图仪测定,分别检测大鼠心脏质量指数、左心室质量指数、左心室舒张期末压(LVEDP)、左心室收缩压(LVSP),取左心室组织进行TUNEL染色,观察组织凋亡率。用RT-PCR检测过氧化体增殖物激活型受体r共激活因子1a(PGC-1a)的表达;Western检测Bax、bcl-2、caspase-3、PGC-1a的表达;ELISE检测血清中AMP、ADP、ATP含量。结果:糖尿病心肌病组的HMI和LVMI分别增加了55%和39%,LVEDP增大了2.2倍,LVSP降低23%;Bax,caspase-3分别降低了1.68倍和1倍,PGC-1a减少76%,bcl-2增加了62﹪;ATP/ADP,ATP/AMP比值分别降低了83%、82%;给药黄芪多糖800mg/kg组与糖尿病心肌病组比Bax,caspase-3表达降低了56%、41%;PGC-1a,bcl-2表达增加了2.5倍,1.3倍;ATP/ADP,ATP/AMP比值升高了4.1倍,4.0倍。结论:黄芪多糖可能通过影响PGC-1a的表达来减轻糖尿病大鼠心肌细胞的凋亡。Objective： To investigate the effects of Astragalus polysaccharide on myocardial cell apoptosis protein in diabetic rats. Methods： 50 SD rats,were randomly divided into control group,diabetic cardiomyopathy group,Astragalus polysaccharide,in low,high dosage treatment group,with each group of ten only. Streptozotocin（ STZ） was injected intraperitoneally to the animals in later four groups to induce type 1 diabetic rats model. Seventh weeks after modeling,control group and diabetic cardiomyopathy group were given pure water,Astragalus polysaccharide,in low,high dosage treatment group were given Astragalus polysaccharide（ 200,400,800 mg / kg / d）. At the end of the 12 thweek,hemodynamic parameters were observed. The one was terminal deoxynucleotidyl transferase-mediated d UTP-biotin nick end labeling assay（ TUNEL） and the other was the expressions of bcl-2,Bax,caspase-3 and PGC-1α of Left ventricular tissue which were measured by Western blot and RT-PCR respectively. Results compared with control group,DM group markedly elevated HMI,LVMI,LVEDP level and decreased LVSP level,meanwhile the expression of Bax were increased and the expressions of PGC-1α,bcl-2 protein decreased. Compared with DM group,the Astragalus polysaccharide of different dose groups dependently improved hemodynamic parameters,ATP / ADP and ATP / AMP ratio,PGC-1α and bcl-2 protein expressions. Conclusions： Apoptosis of Astragalus polysaccharides may reduce the myocardial cells of diabetic rats by affecting PGC-1α expression.

关 键 词：黄芪多糖 心肌细胞凋亡 PGC-1a 能量代量 

分 类 号：R285.5[医药卫生—中药学]