黄精多糖对糖尿病肾病大鼠肾脏的保护作用  

The protective effect of polygonatum sibiricum polysaccharide on diabetic nephropathy rats

作　　者：付婷婷[1] 王国贤[1] 陈婷婷[1] 张忠英[1] 

机构地区：[1]辽宁医学院药理学教研室,锦州121001

出　　处：《中药药理与临床》2015年第4期123-126,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:探究黄精多糖对STZ所致糖尿病大鼠肾脏的保护机制。方法:将大鼠随机分为5组:对照组、糖尿病组、黄精多糖1000、500、250 mg/kg组,后面4组采用小剂量的链脲佐菌素(Streptozocin,STZ)30 mg/kg尾静脉注射建立糖尿病模型,黄精多糖分别以1000、500、250 mg/kg灌胃12周,检测血糖、血肌酐及尿素氮;MASSON染色观察大鼠肾脏形态学变化;考马斯亮兰检测大鼠24小时蛋白尿;免疫组织化学、Western Blot方法测定肾脏组织转化生长因子β1(TGF-β1)、内皮素-1(ET-1)、型胶原(Col-I)蛋白表达。结果:与对照组比较,糖尿病组大鼠血糖及24小时尿蛋白明显增加,肾脏组织中TGF-β1、ET-1、Col-I蛋白表达明显升高;与糖尿病组比较,黄精多糖1000、500、250 mg/kg组血糖及24小时尿蛋白明显降低,肾脏组织中TGF-β1、ET-1、Col-I蛋白表达明显降低。结论:黄精多糖对糖尿病大鼠肾脏有保护作用,其机制可能与其降糖、抑制纤维化因子有关。Objective： To explore the protective effects of polygonatum sibiricum polysaccharide（ PSP） on myocardial tissue in type diabetic rats induced by STZ. Methods： SD rats were randomly divided in to five groups as follows ： the control group,diabetic model group,PSP1000,500,250 mg/kg groups. To establish the type diabetes model,small dose of streptozocin 30mg/kg in the tail veins was used. PSP1000,500,250 mg / kg groups was given ig for 12 weeks in the three PSP treatment groups. Fasting blood glucose serum creatinine（ SCR） and blood urea nitrogen（ BUN） was examined; MASSON staining observes morphological changes in the rat kidney; Bradford detects 24-hour urinary protein in rats. Protein expressions of nephropathy tissue TGF-β1,ET-1 and Col-I were also tested by immunohistochemistry and Western Blot method. Results： Compared with the control group,the levels of blood glucose and 24-hour urinary protein in diabetes model rats were increased significantly（ P〈0. 05）,TGF-β1,ET-1 and Col-I protein expression increased significantly（ P〈0. 05）. Compared with diabetes model group,the levels of blood glucose and 24 hours urinary protein of PSP1000,500,250 mg / kg groups reduced significantly（ P〈0. 05）,the protein expressions of TGF-β1,ET-1 and Col-I were also significantly reduced（ P〈0. 05）. Conclusion： The protective effects of PSP was demonstrated on nephropathy tissue in diabetic rats. Its mechanism is partly associated with lowering blood glucose,fibrosis factors involved and so on.

关 键 词：黄精多糖 糖尿病肾病 转化生长因子Β1 内皮素-1 型胶原蛋白 

分 类 号：R285.5[医药卫生—中药学]