宫颈癌HeLa细胞抗顺铂作用的Notch1机制研究  

Notch1 Mediates the Anti- cisplatin Character of Cervical Cancer Le La Cell Line

作　　者：杨晶[1] 张军红[2] 马慧娟[1] 王志华[1] 王琼[1] 王川[3] 

机构地区：[1]河北医科大学生理学教研室,石家庄050017 [2]河北省邢台市眼科医院病理科、河北省眼病治疗中心、河北省眼科研究所,054000 [3]河北医科大学药理学教研室

出　　处：《医学研究杂志》2015年第10期34-37,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(31171097);国家自然科学基金资助项目(青年项目)(81100229);河北省自然科学基金资助项目(C2014206419);河北省高等学校科学技术研究项目(ZD2015007);邢台市科技支撑计划项目(2013ZC062);河北省卫生厅医学研究重要课题(20090319);河北省科技厅科学技术研究与发展计划项目(11246125D)

摘　　要：目的利用宫颈癌He La细胞系探讨宫颈癌的抗化疗机制。方法利用球形成试验(sphere forming assay)和Western blot法检测顺铂对癌症干细胞形成的影响以及Notch1在He La细胞系的表达。基因沉默试验分析Notch1基因是否在宫颈癌He La癌症干细胞中发挥关键作用。结果顺铂能促进球结构(意味着癌症干细胞形成)的形成。5μmol/L顺铂较1μmol/L顺铂能促进更多球结构形成(P<0.05)。10μmol/L顺铂会抑制球结构形成。5μmol/L顺铂还能提高He La中Notch1的表达(P<0.05),而沉默He La细胞的Notch1后,He La细胞中形成的球数量降低1.7倍(P<0.05)。结论宫颈癌He La细胞系具有抗顺铂治疗的特性,尤其是5μmol/L的顺铂,该抗化疗作用与宫颈癌He La细胞中的Notch1表达升高,最终促进癌症干细胞自我更新有关。Objective To explore the anti -chemotherapy mechanism of cervical cancer using the cervical cancer HeLa cell line . Methods Sphere forming assay and Western blotting were used to measure the potential effects of Cisplatin on cancer stem cell formation and Notch1 expression in HeLa cells .Gene silencing assay was performed to analyze whether Notch 1 played a key role in the proliferation of HeLa cancer stem cells .Results Cispaltin basically promoted the formation of ball structures （ an indicator of cancer stem cells ） ex-cept at larger concentration （10μmol/L）.Cispaltin at 5μmol/L exerted greater promoting effect on the ball structures compared with that at 1μmol/L （P〈0.05）.Cispaltin at 10μmol/L inhibited the formation of ball structures .Cispaltin at 5μmol/L also promoted Notch1 ex-pression in HeLa cells （P〈0.05）, while silencing Notch1 decreased ball structure formation by 1.7 folds （P〈0.05）.Conclusion Cervical cancer HeLa cell line behaves an character of anti -Cispaltin therapy especially at 5μmol/L, and this tendency is likely associat-ed the promoting effect of Cisplatin on the expression of the self -renewal factor Notch1 and proliferation of cancer stem cells .

关 键 词：癌症干细胞Notchl 宫颈癌HeLa细胞系 

分 类 号：R339.2[医药卫生—人体生理学]