儿茶素类化合物巯乙胺基取代物的制备  

Preparation of the cysteamine group substitutes of catechin compounds

作　　者：李姝靓 孙磊[2] 乔善义[2] 常新全 杨桂秋[1] 

机构地区：[1]沈阳化工大学制药与生物工程学院,沈阳110142 [2]军事医学科学院毒物药物研究所,北京100850 [3]中国药材公司,北京102600

出　　处：《国际药学研究杂志》2015年第5期630-633,共4页Journal of International Pharmaceutical Research

基　　金：国家"重大新药创制"科技重大专项资助项目(2011ZX09201-201-13;2012ZX09301003-001-004;2012ZX09301003-001-010)

摘　　要：目的以威麦宁原料药为起始原料,制备儿茶素类化合物巯乙胺基取代物。方法以盐酸巯乙胺为硫解试剂,将威麦宁原料药硫解后,采用C18常压柱色谱分离结合半制备高效液相色谱分离的方法制备儿茶素类化合物巯乙胺基取代物。结果制备得到4β-(2-氨基乙硫基)儿茶素(Cya-Cat)、4β-(2-氨基乙硫基)表儿茶素(Cya-EC)、4β-(2-氨基乙硫基)表儿茶素没食子酸酯(Cya-ECG),纯度均≥95%。讨论该方法步骤简单,操作简便,为儿茶素类化合物巯乙胺基取代物的制备提供了参考。Objective To prepare cysteamine group substitutes of catechin compounds from Weimaining. Methods After thiolased by cysteamine,Weimaining was subjected to the open column chromatography on C18,and subsequently to the semipreparative high performance liquid chromatograph（HPLC） to prepare the cysteamine group substitutes of catechin compounds.Results Compounds 4β-（2-aminoethylthio）catechin,4β-（2-aminoethylthio）epicatechin,and 4β-（2-aminoethylthio） epicatechin-3-O-gallate were obtained,and their purities were above 95%. Conclusion The method is simple and easy,and provides a reference for the preparation of the cysteamine group substitutes of catechin compounds.

关 键 词：威麦宁硫解产物 儿茶素类化合物巯乙胺基取代物 制备 

分 类 号：R284[医药卫生—中药学]