流动注射化学发光法测定注射用比阿培南  

Flow injection chemiluminescence determination of biapenem for injection

作　　者：翟倩[1] 李晓璐[1] 俞宏松 郭晶[1] 易钢[1] 

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016

出　　处：《国际药学研究杂志》2015年第5期642-645,共4页Journal of International Pharmaceutical Research

摘　　要：目的建立一种快速、灵敏测定比阿培南的新方法。方法在碱性介质中,比阿培南对铁氰化钾氧化鲁米诺化学发光体系具有明显的增敏作用,且光信号的增强值与比阿培南的浓度在一定的范围内成正比,据此结合流动注射技术建立了一种测定比阿培南的新方法。结果在最优实验条件下,化学发光增加强度与比阿培南的浓度在0.02~0.6 mg/L范围内呈良好的线性关系,检出限为7.3×10-3 mg/L。对0.2 mg/L比阿培南连续平行测定11次,测得其相对标准偏差为1.76%。结论该方法灵敏度高,检测限低,重复性好,并可用于注射用比阿培南针剂的测定,结果令人满意。Objective To establish a new method for the rapid and sensitive determination of biapenem. Methods A simple,rapid and sensitive flow injection chemiluminescence（CL）method for the determination of biapenem was developed based on the fact that biapenem could enhance the CL of the luminol（LMN）-potassium ferricyanide reaction system under an alkaline condition. Results Under the optimal experimental conditions,we obtained a linear relationship between the CL intensity and the concentration of biapenem in the range of 0.02~0.6 mg/L with a detection limit of 7.3×10-3mg/L. The relative standard deviation was1.76% for the eleven-time determination of 0.2 mg/L biapenem. Conclusion The method shows high sensitivity,low detection limit and good repeatability. It has been successfully applied to the determination of biapenem for injection and the results are satisfactory.

关 键 词：流动注射 化学发光 比阿培南 鲁米诺 铁氰化钾 

分 类 号：R927[医药卫生—药学]