羊棘球蚴(包虫)间接ELISA抗体检测试剂盒的研制  

The Development of the Indirect ELISA Test Kit for Echinococcus granulosus Antibody

作　　者：邓灿新[1] 田东升[1] 李春燕 赵扬扬 曾仁权[1] 曹政 

机构地区：[1]西南大学荣昌校区动物医学系,重庆402460 [2]重庆澳龙生物制品有限公司,重庆402460

出　　处：《安徽农业科学》2015年第30期105-108,共4页Journal of Anhui Agricultural Sciences

基　　金：重庆市农业科技成果转化资金项目(cstc2015jcsf-nycgzh A10002)

摘　　要：[目的]研制基于Eg95重组蛋白为检测抗原的羊棘球蚴(包虫)间接ELISA抗体检测试剂盒。[方法]以细粒棘球蚴Eg95重组蛋白为检测抗原,筛选间接ELISA的最佳反应条件,确定ELISA的判定标准,检测敏感性和重复性,组成、包装试剂盒并初步应用。[结果]建立的ELISA方法特异性强、敏感性高、重复性好,检测阳性血清和阴性血清的准确率均为100%。[结论]研制的羊棘球蚴(包虫)病ELISA抗体检测试剂盒可用于细粒棘球蚴Eg95亚单位疫苗抗体水平的检测。[ Objective] This study was designed to develop an indirect ELISA kit for detecting antibodies against Echinococcus granulosus using recombinant EG95 protein as antigen. [ Method ] Eg95 recombinant protein as antigen, determined the optimal reaction conditions and criterion of indirect ELISA ,detection sensitivity, repeatability, detected its sensitivity and repeatability. [ Result] The ELISA method is high specificity, sensi- tivity,accuracy（ 100% ） and good repeatability. [ Conclusion ] This ELISA kit could be used to monitor the immune status of the echinococcosis Eg95 genetic engineering subunit vaccine.

关 键 词：细粒棘球蚴 Eg95蛋白 试剂盒 ELISA 

分 类 号：S859.795[农业科学—临床兽医学]