转DREB1A/Bar双价基因马铃薯的耐旱性及除草剂抗性分析  

Analysis of drought tolerance and herbicide resistance in transgenic potato plants over-expressing DREB1A/Bar

作　　者：贾小霞[1,2] 齐恩芳[1,2] 马胜[1,2] 胡新元[1,2] 王一航[1,2] 文国宏[1,2] 龚成文[1,2] 李建武[1,2] 

机构地区：[1]甘肃省农业科学院马铃薯研究所甘肃省马铃薯种质资源创新工程实验室,甘肃兰州730070 [2]农业部西北旱作马铃薯科学观测实验站,甘肃渭源748201

出　　处：《草业学报》2015年第11期58-64,共7页Acta Prataculturae Sinica

基　　金：甘肃省自然科学基金(145RJZA088);国家自然科学基金(31060200);甘肃省农业科学院中青年基金(2014GAAS20);国家马铃薯产业技术体系(CARS-10-P05)资助

摘　　要：在前期获得DREB1A/Bar双价转基因马铃薯的基础上,对转基因植株进行了耐旱性和除草剂抗性分析。耐旱性分析显示,在正常浇水条件下,对照和各转基因马铃薯株系生长状态良好且大致相同,各株系的丙二醛含量、相对电导率和SOD酶活性无显著差异(P>0.05)。经过控水10d后,非转基因对照植株叶片明显萎蔫卷曲,而转基因植株仍然保持良好的生长状态;转基因株系的丙二醛含量和相对电导率显著低于非转基因株系(P<0.05),而SOD酶活性显著高于非转基因对照(P<0.05)。控水18d时,大部分对照植株死亡,死亡率为74.33%;转基因植株只有极少数植株死亡,DR2和DR5的死亡率分别为20.43%和5.65%。用0.3%的市售草铵膦喷施各株系,10d后,对照植株全部枯死,转基因株系的个别叶片干枯,绝大多数叶片及所有茎秆生长状态良好。以上分析表明,DREB1A和Bar基因的导入,明显增强了转基因马铃薯对干旱和除草剂的抗性。In order to compare drought resistance of DREB1A/Bar transgenic potato plants,with a control cul-tivar (non-transgenic Longshu 10),5 -7 cm seedlings of DREB1A transgenic lines were grown in pots using vermiculite and regularly watered.Watering continued to the 15-16 leaf stage after which plants were not wa-tered for 18 days to impose for drought stress.During the drought stress,plant phenotypic features were re-corded using visual observation and digital camera images.After 10 days of drought stress,stress-related phys-iological and biochemical parameters were determined.During the normal watering phase both the non-trans-genic control and the transgenic potato lines grew well,the MDA content,relative electrical conductivity and superoxide dismutase (SOD)activity were not significantly different (P >0.05 ).After 10 days of drought stress the control plants began to wilt,but the transgenic lines remained in good condition.MDA content and relative electrical conductivity in the transgenic line was significantly lower than those of the non-transgenic plants (P <0.05),while the SOD activity was significantly higher than that of non-transgenic plants (P <0. 05).After for 18 days of drought stress 74.33% of the control plants were dead whereas fewer transgenic plants had died;the mortality of DR2 and DR5 was 20.43% and 5.65% respectively.The resistance of DREB 1A/Bar transgenic potatoes to glufosinate,a systemic non-selective herbicide,compared with non-trans-genic lines was determined by treating plants with a 0.3% glufosinate spray.Ten days after treatment all non-transgenic plants had died,while the transgenic plants were affected slightly.The study indicated that intro-duction of DREB1A and Bar genes significantly enhanced drought tolerance and herbicide resistance in trans-genic potato plants.

关 键 词：转基因马铃薯 DREB1A BAR 抗旱性 除草剂 

分 类 号：S532[农业科学—作物学]