PEG引发对燕麦老化种子活力的影响  被引量:9

Effects of polyethylene glycol priming on the vigor of aged oat seeds

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作  者:夏方山[1] 闫慧芳[1] 毛培胜[1] 王明亚[1] 郑慧敏[1] 陈泉竹 

机构地区:[1]中国农业大学动物科技学院草业科学北京市重点实验室,北京100193

出  处:《草业学报》2015年第11期234-239,共6页Acta Prataculturae Sinica

基  金:国家十二五科技支撑课题"优质多抗牧草新品种选育与良种繁育关键技术研究与示范"(2011BAD17B01-02)资助

摘  要:为探讨PEG引发对燕麦老化种子活力的影响,并确定其最适浓度和引发时间,试验以45℃老化48d的超干燕麦种子(含水量4%)为材料,通过不同浓度PEG-6000(0,-0.3,-0.6,-0.9和-1.2 MPa)引发0(CK),3,6和12h后,分析其发芽率、发芽指数、平均发芽时间及幼苗活力指数的变化。结果表明:低浓度(0^-0.6 MPa)PEG引发降低了燕麦老化种子的发芽率、发芽指数及幼苗活力指数,并提高了其平均发芽时间;而高浓度(-0.9和-1.2 MPa)则相反。这表明PEG引发不仅能预防超干燕麦种子的吸胀损伤,还能修复其老化损伤,但作用效果与其浓度、引发时间及两者之间交互作用显著相关。该试验中浓度为-1.2 MPa的PEG引发12h时效果最佳,但是否能应用于农业生产实践仍需深入探讨。A study has been undertaken to explore the effects of polyethylene glycol (PEG)priming on the vigor of aged oat seeds (Avena sativa ).The research also aimed to determine the optimal PEG concentration and priming time.Seeds with ultra-dry moisture content (4%)were deteriorated for 48 d at 45℃.They were then primed for 0 (control),3,6 and 12 h with different concentrations of PEG-6000 (0,-0.3,-0.6,-0.9 and-1.2 MPa).The germination percentage,germination index,mean time to germination and seedling vigor in-dex were then analyzed.The results showed that the germination percentage,germination index and seedling vigor index of aged oat seeds decreased after priming with low concentrations of PEG (0--0.6 MPa)in rela-tion to the control,but that mean time to germination increased.However,the opposite trend was observed after priming with high PEG concentrations (-0.9 and -1.2 MPa).These results indicate that PEG priming not only prevents the imbibition damage for oat seeds with ultra-dry moisture content but also repairs damage from seed ageing.The effect of PEG priming was closely related to its concentration,priming time and their interaction.This study determined that the appropriate concentration and priming time was -1.2 MPa and 12 h for aged oat seeds.However,further research is needed to confirm whether these levels can be practically ap-plied in agricultural production.

关 键 词:引发 聚乙二醇 种子老化 超干 燕麦 种子活力 

分 类 号:S512.6[农业科学—作物学]

 

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