冬青叶鼠刺叶绿素的提取及其稳定性  

Extraction and Stability of Chlorophyll from Itea ilicifolia Leaves

作　　者：敖国富[1,2] 周甜敏 张来[1,2] 

机构地区：[1]安顺学院农学院,贵州安顺561000 [2]安顺学院贵州省教育厅功能材料与资源化学特色重点实验室,贵州安顺561000 [3]安顺市第三中学,贵州安顺561000

出　　处：《贵州农业科学》2015年第10期82-85,共4页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目青年项目(81102796);贵州省科技厅;安顺市人民政府;安顺学院联合基金项目[黔科合J字LKA(2012)13];贵州省教育厅功能材料与资源化学特色重点实验室专项[黔教高发(2011)278]

摘　　要：为给冬青叶鼠刺的生理生态研究提供理论参考,采用7种提取溶剂在室温和冰浴条件下提取冬青叶鼠刺的叶绿素,研究在不同光照时间、不同温度、不同浓度H2O2及处理时间和金属离子条件下叶绿素的稳定性。结果表明:在室温条件下,无水乙醇∶丙酮(1∶1)混合液提取冬青叶鼠刺叶绿素a和叶绿素b的吸光度分别为1.622和0.675,80%丙酮提取叶绿素a和叶绿素b的吸光度分别为0.458和0.264,说明无水乙醇∶丙酮(1∶1)提取冬青叶鼠刺叶绿素的效果最佳,80%丙酮的效果最差;H2O2对冬青叶鼠刺叶绿素稳定性的影响较大,温度对冬青叶鼠刺叶绿素稳定性的影响较小,光照时间对冬青叶鼠刺叶绿素a影响大;金属离子K+、Na+、Zn2+和Mg2+对叶绿素a稳定性影响不明显,Al 3+对叶绿素a稳定性减弱,Fe2+、Cu2+和Ca2+对叶绿素a的稳定性减弱。The chlorophyll samples extracted from I. ilicifolia leaves by using seven different extraction solvents under room temperature and ice-bath condition, and then the effect of different illumination time, different temperature, different treatment time of H2O2 with different concentration and different metal ions on stability of chlorophyll samples was studied to provide a theoretical reference for physiological and ecological studies of I. ilicifolia. Results. The absorbance of chlorophyll a and chlorophyll b extracted from I. ilicifolia by the mixed solvent of absolute ethyl alcohol and acetone （1：1） at room temperature is 1. 622 and 0. 675 respectively but the absorbance of chlorophyll a and chlorophyll b extracted from I. ilicifolia by 80% acetone is 0. 458 and 0. 264 separately, which indicates that the mixed solvent of absolute ethyl alcohol and acetone （1： 1） is the best extraction solvent and the 80% acetone solvent is the worst extraction solvent for extracting chlorophyll from I. ilicifolia. H2 02 has a large influence on stability of chlorophyll extracted from I. ilicifolia but temperature has a little influence on stability of chlorophyll extracted from I. ilicifolia. Illumination time has a large influence on stability of chlorophyll a extracted from I. ilicifolia. K＋, Na＋, Zn2＋ and Mg2＋ have no obvious influence on stability of chlorophyll a extracted from I. ilicifolia. But A1a＋ , Fez＋ , Cu2 ＋ and Caz＋ can reduce stability of chlorophyll a extracted from I. ilicifolia.

关 键 词：冬青叶鼠刺 叶绿素 提取 稳定性 

分 类 号：Q946.91[生物学—植物学]