应用1-氨基环丙烷-1-羧酸脱氨酶产生菌提高双孢蘑菇产量  

Application of 1-aminocyclopropane-1-carboxylic Acid Deaminase Producing Bacterium for Increasing the Yield of the Button Mushroom

作　　者：程雁[1,2] 王景冒 张岩[1] 杨潜龙 邱立友[1] 

机构地区：[1]河南农业大学生命科学学院农业部农业微生物酶工程重点实验室,郑州450002 [2]河南省科学院生物研究所有限责任公司,郑州450008

出　　处：《河南科学》2015年第10期1750-1755,共6页Henan Science

基　　金：河南省基础与前沿技术研究计划项目(112300410115)

摘　　要：比较了6种覆土处理对双孢蘑菇产量的影响.6种覆土处理分别是常规覆土、常规覆土＋恶臭假单胞菌UW4菌剂（用量为覆土干重的2%）、灭菌常规覆土、灭菌常规覆土＋UW4菌剂（用量为覆土干重的2%）、灭菌蛭石和灭菌蛭石＋UW4菌剂（用量为蛭石干重的2%）.UW4是常用的植物根际促生细菌1-氨基环丙烷-1-羧酸（ACC）脱氨酶产生菌.结果表明,灭菌蛭石＋UW4菌剂处理出菇最早,产量最高,无杂菌污染,出菇比其他处理提早1-8 d,比常规覆土提早3 d,第一潮和第二潮菇产量之和比其他处理提高19.8%-115%,比常规覆土提高35.1%.采收二潮菇后,不同处理覆土材料中细菌和ACC脱氨酶产生菌的数量均与菇产量呈显著的正相关.本研究结果提示,灭菌蛭石中添加ACC脱氨酶产生菌剂替代传统覆土是实现双孢蘑菇绿色、高效生产的有效方式。In this study, the effects of the six casing treatments on the yield of the button mushroom were compared. The six casing treatments were that soil casing, soil＋Pseudomonas putida UW4 bacterial agent （2% of the soil dry weight）, sterilized soil, sterilized soil＋ UW4 bacterial agent （2% of the soil dry weight）, sterilized vermiculite, and sterilized vermiculite ＋ UW4 bacterial agent （2% of the vermiculite dry weight） , respectively. Pseudomonas putida UW4 is commonly used as plant growth-promoting rhizobacteria and 1-aminocyelopropane-1- carboxylic acid （ACC） deaminase producing bacteria. The results showed that the fruiting bodies emerged earliest （the duration from casing to primordium initiation was earlier 1-8 d） and the yield of the first and second crops was the highest （higher 19.8% to 115%） with not any contamination by casing sterilized vermiculite＋UW4 compared to the other casing treatments. In details, casing of sterilized vermiculite＋UW4 the fruiting bodies emerged earlier 3 d and the yield of the first and second crops was higher 35.1% than that of soil casing. The number of bacteria and ACC deaminase producing bacteria in the casing material after harvest two flush mushrooms and the yield of the first and second crops showed significant positive correlation, respectively. The results of this study suggest that it is a facility procedure for green and efficient production of the button mushroom by using sterilized vermiculite adding ACC deaminase producing bacteria agent instead of the traditional soil casing.

关 键 词：双孢蘑菇 1-氨基环丙烷-1-羧酸脱氨酶 覆土 蛭石 

分 类 号：S646[农业科学—蔬菜学]