北方春玉米区玉米穗腐病禾谷镰孢菌复合种SCAR类型测定  

SCAR type detection of Fusarium graminearum clade species complex on spring maize producing areas of north China

作　　者：董怀玉[1] 王大为[2] 王丽娟[1] 刘可杰[1] 姜钰[1] 徐秀德[1] 左驰 

机构地区：[1]辽宁省农业科学院植物保护研究所,沈阳110161 [2]辽宁省农业科学院玉米研究所,沈阳110161 [3]抚顺市农业技术推广中心,辽宁抚顺113008

出　　处：《东北农业大学学报》2015年第10期23-28,共6页Journal of Northeast Agricultural University

基　　金：国家科技支撑计划项目(2011BAD16B12;2012BAD04B03;2013BAD07B03)

摘　　要：利用禾谷镰孢菌特异性引物Fg16F/R,对分离自我国北方春玉米区玉米穗腐病籽粒病原禾谷镰孢菌复合种(Fus arium gramine arum clade)的45株菌株作SCAR类型测定,并通过基因序列测定解析。结果表明,我国北方玉米穗腐病籽粒中携带的病原禾谷镰孢菌复合种存在两种SCAR类型:即类型1(F.gramine arum)和类型5(F.as iaticum),分别占供试菌株的80%和20%,并以F.gramine arum为优势致病种类。Based on specific primers Fg16F/R of Fusarium graminearum, of 45 F. graminearum clade strains which were isolated from maize seeds of spring maize producing areas of north China were detected for the SCAR types. The results showed that Fusarium graminearum clade were detected two kinds of SCAR types, SCAR type 1 and SCAR type 5, the species were F. graminearum and F. asiaticum, separating rate was 80% and 20%, respectively, F. graminearum was superiority superiority type.

关 键 词：玉米 禾谷镰孢菌复合种 SCAR类型 PCR 

分 类 号：S513[农业科学—作物学]