低温纤维素降解菌分离鉴定及产酶条件优化  

Isolation and identification of low-temperature cellulose-degrading bacterium and optimization of enzyme production conditions

作　　者：李春艳[1] 于琦[1] 冯露[1] 成毅[1] 成小松[2] 王雪[1] 张平根[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030 [2]哈尔滨医科大学第一临床医学院,哈尔滨150001

出　　处：《东北农业大学学报》2015年第10期74-81,共8页Journal of Northeast Agricultural University

基　　金："十二五"国家科技支撑计划项目(2013BAJ12B02-4)

摘　　要：采用富集培养方法在低温条件下从生活垃圾土壤中分离得到1株能够降解纤维素细菌,命名为FLX-1。经形态学、生理生化学和分子生物学16S r DNA序列分析,将菌株FLX-1鉴定为节杆菌属(Arthrobacter sp.)细菌。为探索菌株FLX-1生长特性和最佳产酶条件,利用响应面分析方法考查培养时间、温度、p H和接种量对菌株FLX-1产羧甲基纤维素(Carboxymethyl cellulose,CMC)酶活特性影响情况。结果表明,菌株FLX-1最佳产酶条件为培养时间74.1 h,温度10.5℃,p H 7.1,接种量2.1%。在最佳条件下,菌株FLX-1产CMC酶活值为14.12 U·m L-1。A cellulose-degrading strain FLX-1 was isolated from domestic waste soil samples at low temperature by enrichment culture. According to morphologic and physiological-biochemical characteristics as well as 16 S r DNA sequence analysis, strain FLX-1 was identified as Arthrobacter sp.The effects of culture time, temperature, p H and inoculation size on the carboxymethyl cellulose enzyme activity by strain FLX-1 were optimized using a response surface methodology（RSM）. The results showed that the optimal enzyme production conditions were culture time 74.1 h, temperature10.5 ℃, p H 7.1 and inoculation size 2.1%. At the best conditions, the carboxymethyl cellulose enzyme activity of strain FLX-1 was 14.12 U· m L-1.

关 键 词：纤维素 低温降解菌 Arthrobacte r sp. CMC酶 响应面分析法 

分 类 号：S789[农业科学—林学] S182