健脾解毒方通过抑制MALAT1介导的β-catenin信号通路抗大肠癌侵袭转移  

Effects of Jianpi Jiedu Recipe on the inhibition of invasion and migration of colorectal cancer through MALAT1 mediated β-catenin signaling pathway

作　　者：季青[1] 刘宣[1] 周利红[1] 韩植芬[1] 颜琳琳[1] 江海丽 陈静[1] 任建琳[2] 李琦[1] 

机构地区：[1]上海中医药大学附属曙光医院肿瘤科,上海201203 [2]上海中医药大学附属中医医院肿瘤科,上海200071

出　　处：《中华中医药杂志》2015年第11期3877-3880,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81303102;No.81303103;No.81473478;No.81473628);上海市卫生局项目(No.20114037)~~

摘　　要：目的:探讨健脾解毒方通过抑制MALAT1介导的β-catenin信号通路抗大肠癌侵袭转移的作用机制。方法:利用慢病毒介导的RNA干扰技术沉默MALAT1基因,观察健脾解毒方对大肠癌细胞侵袭转移及MALAT1介导的β-catenin信号通路的调控作用。结果:健脾解毒方能够抑制大肠癌细胞的侵袭转移能力,直接下调MALAT1基因的表达,抑制大肠癌细胞胞核内β-catenin的积累及其下游靶基因MMP-7的表达;MALAT1基因能够增加β-catenin在细胞核内的积累及其下游靶基因MMP-7的表达。结论:MALAT1通过激活β-catenin信号通路并促进下游靶基因MMP-7的表达以达到促进大肠癌细胞侵袭转移的目的,而健脾解毒方能够通过直接抑制MALAT1的表达发挥抗大肠癌细胞侵袭转移的作用。Objective： To investigate the mechanism of Jianpi Jiedu recipe（JPJDR） on the inhibition of the invasion and migration of colorectal cancer through MALAT1 mediated β-catenin signaling pathway. Methods： The lentivirus mediated RNA interfere technology was used to silence the expression of MALAT1. And then, how JPJDR to regulate the β-catenin signaling pathway mediated by MALAT1 and to influence on the invasion and migration of colorectal cancer were observed. Results： JPJDR inhibited the ability of invasive and migratory of colorectal cancer. JPJDR reduced the accumulation of β-catenin in the nuclear of colorectal cancer as well as the expression of MMP-7 gene, and importantly JPJDR directly down-regulated the expression of MALAT1 gene, which could increase the accumulation of β-catenin in the nuclear and promote the expression of β-catenin targeted MMP-7 gene. Conclusion： Jianpi Jiedu recipe could inhibit the invasion and migration of colorectal cancer through MALAT1 mediated β-catenin signaling pathway.

关 键 词：健脾解毒方 MALAT1 Β-CATENIN 侵袭转移 大肠癌 

分 类 号：R285[医药卫生—中药学]