葛根素对破骨细胞形成以及成骨细胞OPG/RANKL mRNA表达的影响  

Effects of puerarin on the formation of osteoclasts and the expression of OPG/RANKL mRNA of osteoblasts

作　　者：袁斯远 何芳[1] 孔蓓蓓[2] 刘连起[2] 张允岭[1] 马涛[1] 刘雪梅[1] 郑宏[1] 闫妍[1] 王新祥[1] 

机构地区：[1]北京中医药大学东方医院实验中心,北京100078 [2]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2015年第11期3889-3892,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30873290)~~

摘　　要：目的:观察葛根素对破骨细胞的形成以及成骨细胞OPG、RANKL mRNA表达的影响。方法:通过培养小鼠破骨前驱细胞RAW 264.7诱导产生破骨细胞,在培养液中分别加入葛根素(10-5、10-6、10-7mol/L),利用TRAP染色观察破骨细胞形成数目。通过培养小鼠成骨细胞MC3T3-E1,在培养液中加入葛根素(10-6mol/L),利用RT-PCR方法观察成骨细胞OPG、RANKL mRNA的表达。结果:葛根素组破骨细胞形成数目随着葛根素浓度增加而显著减少(P<0.05)。与空白对照组比较,葛根素组成骨细胞OPG mRNA表达升高,但差异无统计学意义,RANKL mRNA表达降低(P<0.05),OPG/RANKL mRNA升高。结论:葛根素能够直接抑制破骨细胞形成和上调成骨细胞OPG/RANKL mRNA表达而间接地调控破骨细胞,可能是葛根素抗骨吸收作用的重要细胞分子机制。Objective： To observe the effects of puerarin on the formation of osteoclasts and the expression of OPG and RANKL m RNA of osteoblasts. Methods： The mouse osteoclast precursor cells RAW 264.7 were cultivated to induce osteoclasts. During experiment, puerarin at 10^-5, 10^-6, 10^-7mol/L were respectively added into the culture solution. Then, the TRAP staining was used to observe the number of osteoclast. Meanwhile, the mouse osteoblasts cells MC3T3-E1 were cultivated. The puerarin at 10-6mol/L was added into the culture solution. RT-PCR method was used to detect the expression of OPG and RANKL m RNA. Results： With an increase of puerarin concentration, the number of osteoclast significantly reduced. Compared with the control group, the expressions of OPG m RNA in puerarin groups were all higher, while the RANKL m RNA in puerarin groups lower（P〈0.05）, the ratio of OPG to RANKL increased in puerarin groups. Conclusion： Puerarin could directly inhibit the formation of osteoclast and indirectly regulate osteoclast through up-regulating the ratio of OPG to RANKL m RNA, which might be an important cellular and molecular mechanism of puerarin on anti-bone resorption.

关 键 词：葛根素 OPG RANKL 破骨细胞 成骨细胞 

分 类 号：R285.5[医药卫生—中药学]