参麦汤对糖耐量低减大鼠肿瘤相关因子SF及TNF-α的影响  

Influence of Shenmai Decoction on tumor related factors SF and TNF-α in impaired glucose tolerance rats

作　　者：谷英敏[1,2] 柴可夫[1] 朱玮华 王磊[1] 王玉娟[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]天台人民医院,台州317300

出　　处：《中华中医药杂志》2015年第11期3910-3914,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2012年浙江省中医药科学研究基金计划(No.2012ZA133);台州市科技计划项目(No.1201Ky51);国家自然科学基金项目(No.81273615)~~

摘　　要：目的:研究参麦汤对糖耐量低减(IGT)大鼠肿瘤相关因子肿瘤坏死因子-α(TNF-α)、血清铁蛋白(SF)的影响及二者与糖脂代谢的相关性,探讨参麦汤的作用机制及IGT的病理机制。方法:复制IGT动物模型,用参麦汤治疗,以二甲双胍作对照。采用Elisa法及酯酶法检测IGT大鼠血清SF、TNF-α,甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。结果:模型组比正常组TNF-α、SF、TG、TC、LDL-C升高,HDL-C降低,差异有统计学意义(P<0.05,P<0.01);各干预组均能降低TNF-α、SF水平,其中以参麦汤作用明显(TNF-α、SF,P<0.01),运动组(TNF-α、SF,P<0.01)、二甲双胍组(SF,P<0.01)次之。相关性分析:SF与FPG(r=0.677,P<0.01)、TNF-α(r=0.810,P<0.01)、TG(r=0.539,P<0.01)水平呈显著正相关,与HDL-C(r=-0.364,P<0.01)呈显著负相关;TNF-α与FPG(r=0.700,P<0.01)、TG(r=0.467,P<0.01)、TC(r=0.323,P<0.05)水平呈显著正相关。结论:参麦汤可降低IGT大鼠SF和TNF-α的水平;SF和TNF-α可能参与了糖脂毒性的发生;参麦汤逆转IGT、改善糖脂毒性可能与其调节SF和TNF-α的水平有关。Objective： To study the influence of Shenmai Decoction on tumor related factors serum ferritin（SF） and tumor necrosis factor-α（TNF-α） in impaired glucose tolerance（IGT） rats, and the correlation among TNF-α, SF and glucolipid metabolism in order to investigate the mechanism of Shenmai Decoction and the pathogenic mechanism of IGT. Methods： IGT rat models were established, and then the rats were treated with Shenmai Decoction with metformin as the control therapy. Elisa and Ester enzymatic method were used to measure the levels of TNF-α, SF, TG, TC, HDL-C and LDL-C in serum of IGT rats. Results： The levels of TNF-α, SF, TG, TC and LDL-C in serum of rats in model group were higher than that of control group, while the HDL-C level of rats in model group was lower than that of control group, and the difference was statistical significance（P〈0.05, P〈0.01）. The levels of TNF-α and SF of rats in each intervention group were all reduced, and the intervention effect of Shenmai Decoction was the most significant（P〈0.01）, and followed by motion group and metformin group（P〈0.01）. The results of correlation analysis showed that there was a positive correlation between SF levels and levels of FPG（r=0.677, P〈0.01）, TNF-α（r=0.810, P〈0.01） and TG（r=0.539, P〈0.01）, and there was a negative correlation between SF levels and HDL-C levels（r=--0.364, P〈0.01）. TNF-α levels were correlated positively with levels of FPG（r=0.700, P〈0.01）, TG（r=0.467, P〈0.01）, and TC（r=0.323, P〈0.05）. Conclusion： Shenmai Decoction could decrease the levels of SF and TNF-α of IGT rats, which might participate in the occurrence of glucolipotoxicity. Shenmai Decoction could reverse the progress of IGT and improve the glucolipotoxicity, and the mechanism might relate with its regulation in levels of SF and TNF-α.

关 键 词：参麦汤 糖耐量低减 肿瘤坏死因子-Α 血清铁蛋白 

分 类 号：R285.5[医药卫生—中药学]