蛋氨酸脑啡肽小鼠体内抗癌活性初探  

Anti-tumor Activity of Methionine-enkephalin(MEK)in Mice In Vivo

作　　者：刘璐[1] 游如旭[2] 单风平[3] 曾凡波[2] 贺国芳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]华中科技大学同济医学院药学院药物研究中心,武汉430030 [3]中国医科大学基础医学院免疫学教研室,沈阳110001

出　　处：《华中科技大学学报（医学版）》2015年第5期524-527,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的研究蛋氨酸脑啡肽(MEK)是否具有抗癌、增加化疗药物疗效和增强或保护免疫功能的作用。方法 BALB/c小鼠40只,右腋皮下接种H22癌细胞,接种24h后随机分为5组,依次为阴性对照组(生理盐水0.2mL/kg)、5-Fu组(10mg/kg)、MEK组(200mg/kg)、5-Fu+MEK联合给药组(10mg/kg+200mg/kg)和阳性对照组(20mg/kg 5-Fu)。腹腔注射受试药品,每日1次,连续给药8d,处死动物。计算肿瘤抑制率、胸腺和脾脏脏器指数以及测定CD3+和CD4+T细胞表达。结果单独给予MEK 200mg/kg或5-Fu 10mg/kg时两组对肿瘤的抑制作用相近,对肿瘤的抑制率分别为75.13%和74.26%;联合给予MEK和5-Fu时,其抑瘤率为88.25%,明显高于单独使用5-Fu。MEK对小鼠脾脏及胸腺无明显抑制作用,与5-Fu合用时能显著减轻5-Fu对免疫脏器胸腺的抑制作用。单独给予MEK 200mg/kg时,能明显提高荷瘤小鼠的CD3+和CD4+T细胞表达,与阴性对照组和5-Fu各剂量组比较差异均有统计学意义(均P<0.05)。结论 MEK具有良好的抗肿瘤活性及增强机体免疫功能的作用,与化疗药物合用能增强其疗效同时减轻其毒性,对免疫脏器有保护作用。Objective To study the anti-tumor,chemotherapy-sensitizing and immune-enhancing roles of methionine-enkephalin（MEK）.Methods Experimental BALB/c mice were subcutaneously inoculated with H22 tumor cells in right axillary regions.They were divided into 5groups at random：negative control group（0.2mL/kg NaCl）,5-Fu group（10mg/kg 5-Fu）,MEK group（200mg/kg MEK）,5-Fu＋MEK group（10mg/kg 5-Fu＋200mg/kg MEK）and positive control group（20mg/kg5-Fu）.Animals in each group were intraperitoneally injected with agents once a day for 8days after inoculated with tumor cells24 hlater.The anti-tumor rate,organ indexes of the thymus and spleen and the proportion of CD3＋/CD4＋were calculated.Results The anti-tumor rate was 75.13% in MEK group and 74.26% in 5-Fu group and there was no significant difference.It was 88.25%in 5-Fu＋MEK group,significantly higher than in 5-Fu group.MEK had no significant inhibition on the spleen and thymus.The inhibitive effect of 5-Fu on the thymus and spleen was significantly attenuated after administration with MEK.Moreover,MEK could enhance the proportion of CD3＋and CD4＋significantly（P〈0.05）,and statistical difference was noted between MEK group and control or 5-Fu group.Conclusion MEK has remarkable anti-tumor activity and it can booster immune functions,enhance the efficacy of chemotherapeutics while reducing their toxicity when co-administered.Meantime,it could protect the immune organs.

关 键 词：蛋氨酸脑啡肽 抑制肿瘤 减毒增效 免疫功能 

分 类 号：R979.1[医药卫生—药品]