重组人促红细胞生成素对脑白质损伤3日龄鼠胶质纤维酸性蛋白表达的影响  

Effects of recombinant human erythropoietin on the expression of GFAP in P3 neonatal rats with white matter damage

作　　者：齐俊冶 宋燕燕[1] 周卓妍[2] 蔡岳鞠[1] 陈美苑[2] 肖旭文 王兰秀[1] 

机构地区：[1]广州医科大学附属广州市妇女儿童医疗中心新生儿科,广东广州510623 [2]暨南大学医学院生理学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2015年第5期410-416,共7页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省科技计划项目(2012B061700010)

摘　　要：目的:探讨重组人促红细胞生成素(rh EPO)对3日龄大鼠缺氧缺血性脑白质损伤的胶质纤维酸性蛋白(GFAP)表达的影响.方法:150只3日龄(P3)SD大鼠随机分为对照组,脑室周围白质软化(PVL)组和r EPO治疗组.于造模后1、7、21 d对各组大鼠脑质量损伤百分比进行检测,造模后3、7、14、21 d用免疫组织化学方法观察脑组织GFAP表达情况等来研究EPO对3日龄大鼠缺血缺氧性脑白质损伤的保护作用.结果:PVL组和r EPO组的脑质量损伤%在各取样时间点均明显大于对照组(P<0.05),且r EPO组明显小于PVL组(P<0.05).HE染色示PVL组术后7 d胼胝体结构疏松,术后21 d可见侧脑室扩大明显;r EPO组术后7 d胼胝体结构基本正常,术后21 d侧脑室仅稍微扩大.术后3 d PVL组GFAP阳性表达增多(P<0.05),术后7 d r EPO组和PVL组GFAP阳性表达均明显增多(P<0.05),且PVL组增多更为明显(P<0.05);术后14 d r EPO组与PVL组GFAP阳性表达开始回落,但仍多于对照组(P<0.05),PVL组阳性表达数仍然比r EPO组多(P<0.05),术后21 d各组GFAP阳性表达数基本回落至正常水平.结论:脑白质损伤时GFAP阳性表达增加,r EPO能减少脑白质损伤后GFAP阳性表达.Aim：To explore the neuroprotection effects of recombinant human erythropoietin（rEPO） on 3 day-old neonatal rats with white matter damage.Methods：150 three day-old Sprague-Dawley（SD） rats were randomly divided into control,periventricular leukomalacia（PVL）and rEPO treatment groups （each group n =50）.The brain weight and percentage of the brain damage were recorded at 1 d、7 d and 21 d post operation.Immunohistochemistry method was used to determine the changes of GFAP expres-sion levels in the brain at 3 d、7 d 、14 d and 21 d.Results：The percentage of brain damage in the rEPO and PVL groups was found to be significantly increased compared to the control group（P 〈0.05）at each sampling time point,but that of rEPO group was less than PVL group（P 〈0.05）;Compared to the con-trol group,HE staining showed that in the rEPO group the structure of callosum was basically normal at 7 d post-operation but the lateral ventricles became mildly expanded at 21 d post-operation.However,a loose callosum structure and dilated lateral ventricles were observed at the same sampling time point in PVL group;The expression of GFAP in PVL group was found to be increased at 3 d post-operation（P 〈0.05）.The expression of GFAP in both rEPO and PVL groups increased significantly compared to that of the control group 7 d after modeling,especially in PVL group（P 〈0.05）.The expression of GFAP in both rEPO and PVL groups increased slightly but still higher than that of the control group（P 〈0.05）,and GFAP expression level in PVL group was higher than that of rEPO group（P 〈0.05）14 d after modeling. At 21 d post-operation,the expression of GFAP in each group recovered to normal levels.Conclusion：The expression of GFAP increased after damage of the white matter.rEPO treatment can significantly re-duce the expression level of GFAP after the white matter damage.

关 键 词：重组促红细胞生成素 脑室周围白质软化 胶质纤维酸性蛋白 缺血缺氧 

分 类 号：R722.1[医药卫生—儿科] R338.2[医药卫生—临床医学]