In Vitro Propagation of Ardisia mamillata Hance  

作　　者：Bihua CHEN Juan ZHANG Zhuoxi WU Huihua FAN Qianzhen LI 

机构地区：[1]Fujian Academy of Forestry Sciences [2]Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China,China Forestry Bureau [3]Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China,China Forestry Bureau

出　　处：《Agricultural Science & Technology》2015年第10期2159-2161,2166,共4页农业科学与技术（英文版）

基　　金：Supported by Fujian Modern Agriculture Project:The Innovation and Industrialization Techniques of Dominant Woody Flowering Plants(No.:Min Lin Ji Cai[2012]137)

摘　　要：Ardisia mamillata Hance is a rare plant with highly ornamental and medicinal value. The traditional propagation methods for A. mamillata by seeds or cutting provided low proliferation rate. This study is to optimize the propagation technique of A. mamillata by tissue culture and set up an industrial production system to provide plenty of A. mamillata seedlings for the human demand. The optimal initiation medium for A. mamillata is MS +2.0 mg/L BA +0.1 mg/L NAA +30 g/L sugar, providing76.4% initiation rate. The optimal shoot proliferation medium for A. mamillata is MS+1.0 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 4.56 fold proliferation rate and3.10 cm shoot in height. The optimal shoot elongation medium for A. mamillata is MS+0.5 mg/L BA+0.1 mg/L NAA+30 g/L sugar, providing 2.77 fold proliferation rate and 4.27 cm shoot in height. The optimal rooting medium for A. mamillata is 1/2MS+0.1 mg/L IBA +15 g/L sugar, providing 99.7% rooting rate, 4.0 roots per individual,7.53 cm root in length and 3.94 cm shoot in height. This provides a reliable mass propagation method for A. mamillata.Ardisia mamillata Hance is a rare plant with highly ornamental and medicinal value. The traditional propagation methods for A. mamillata by seeds or cutting provided low proliferation rate. This study is to optimize the propagation technique of A. mamillata by tissue culture and set up an industrial production system to provide plenty of A. mamillata seedlings for the human demand. The optimal initiation medium for A. mamillata is MS ＋2.0 mg/L BA ＋0.1 mg/L NAA ＋30 g/L sugar, providing76.4% initiation rate. The optimal shoot proliferation medium for A. mamillata is MS＋1.0 mg/L BA＋0.1 mg/L NAA＋30 g/L sugar, providing 4.56 fold proliferation rate and3.10 cm shoot in height. The optimal shoot elongation medium for A. mamillata is MS＋0.5 mg/L BA＋0.1 mg/L NAA＋30 g/L sugar, providing 2.77 fold proliferation rate and 4.27 cm shoot in height. The optimal rooting medium for A. mamillata is 1/2MS＋0.1 mg/L IBA ＋15 g/L sugar, providing 99.7% rooting rate, 4.0 roots per individual,7.53 cm root in length and 3.94 cm shoot in height. This provides a reliable mass propagation method for A. mamillata.

关 键 词：Ardisia mamillata In vitro PROPAGATION MEDIUM Red-core soil 

分 类 号：S567.19[农业科学—中草药栽培]