Establishment of Rapid Propagation Technique for Cotyledon of Solanum torvum  

水茄子叶培养简化快繁技术的建立（英文）

作　　者：王益奎[1,2] 李文嘉[1] 蒋雅琴[1] 黎炎[1] 吴永官[1] 康德贤[1] 方岩岩[1] 

机构地区：[1]广西农业科学院蔬菜研究所,广西南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,广西南宁530007

出　　处：《Agricultural Science & Technology》2015年第10期2167-2169,2188,共4页农业科学与技术（英文版）

基　　金：Supported by the"Twelfth Five Year Plan"National Science and Technology Plan Project of Rural Areas in China(2012AA100103007);the Transformation Projects of National Agricultural Science and Technology Achievements(2013GB2E100381);the Guangxi Innovation Team Project of Staple Vegetable of Modern Agricultural Industry Technology System(nycytxgxcxtd-03-10);the Science and Technology Planning Project of Guangxi(14123006-35,14123004-3-5);the Special Fund for Basal Research in Guangxi Academy of Agricultural Sciences(2012YT05,2015YT67)~~

摘　　要：[Objective] To research the mass propagation system for cotyledon of Solanum torvum. [Methods] With cotyledon of S. torvum as the research object, ef- fects of hormone combination on callus induction and adventitious buds differentia- tion of S. torvum were researched. [Results] With cotyledon of S. torvum as the ex- plants, the optimal culture medium for callus induction and adventitious buds differ- entiation was MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA. The induction rates of callus and adventitious bud reached 100% and 85%, respectively. The number of average buds was 6. The optimal culture medium for the induction of adventitious roots was MS＋ 0.3 mg/L IAA. The rooting rate reached 100% and the number of average roots was 9. [Conclusions] One-step induction of callus and adventitious buds simplified the mass propagation system, and enhanced the test test efficiency.s[目的]研究简化水茄子叶培养快繁技术体系试验流程。[方法]以水茄子叶为对象,研究不同激素浓度处理对其愈伤组织的诱导、增殖、分化及生根数的影响。[结果]以水茄子叶为外植体,诱导愈伤组织和不定芽的最佳培养基均为MS+2.0 mg/L 6-BA+0.3 mg/L NAA,愈伤组织诱导率高达100%,不定芽诱导率为85%,平均芽数6个。诱导水茄不定根的最佳培养基为MS+0.3 mg/L IAA,生根率达到100%,平均根数9条。[结论]一步诱导水茄诱导愈伤组织和不定芽方法简化了快繁技术体系,提高试验效率。

关 键 词：Solanum torvum Plant Reqeneration CALLUS Adventitious bud 

分 类 号：S567.19[农业科学—中草药栽培]