Isolation and Identification of the Pathogen Causing Skin Ulcer Disease in Cynoglossus semilaevis Günther and Its Sensitivity to Chinese Herbal Medicine  

半滑舌鳎腐皮病病原分离鉴定及对中草药的敏感性实验（英文）

作　　者：高桂生[1] 张艳英[1] 史秋梅[1] 高光平[1] 韩红升 黄晓媛 缴宝丰 常瑶瑶 

机构地区：[1]河北省预防兽医学重点实验室河北科技师范学院动物科技学院,河北秦皇岛066600 [2]秦皇岛粮丰海洋生态科技股份有限公司,河北秦皇岛066600

出　　处：《Agricultural Science & Technology》2015年第10期2221-2224,2276,共5页农业科学与技术（英文版）

基　　金：Supported by Post-award Grants Program of Science and Technology Department of Hebei Province(15926620H);Science and Technology Support Program of Qinhuangdao Municipal Bureau of Science and Technology(201401A067,201402B043)~~

摘　　要：In this study, the pathogen causing skin ulcer disease in Cynoglossus semilaevis Gunther was isolated for morphological observation, physiological and bio- chemical identification. According to the result, the isolated pathogen was identified as Vibrio harveyi. The results of recurrent infection of C. semilaevis Gonther showed that the pathogen was strongly pathogenic to C. semilaevis Gunther. In or- der to explore the pathogenesis, outer membrane protein （OMP） gene of C. semi- laevis Gunther was detected by PCR. The results showed that all the three repre- sentative strains harbored OMP gene. According to the results of sensitivity test of the pathogen to Chinese herbal medicine, Galla Chinensis, Fructus Mume, Fructus Hippophae and Lignum Sappan exerted strong antibacterial effects against V. harveyr, Pericarpium Granati exhibited slight antibacterial effect against V. harveyi; Pericarpium Citri Reticulatae, Rhizoma Acori GramineL Herba Houttuyniae, Herba Portulacae, Herba Andrographis, Eucalyptus globulus Labill. and Herba Menthae Heplocalycis had little effect on V. harveyi. Galla Chinensis, Fructus Mume, Fructus Hippophae and Lignum Sappan were prepared into three prescriptions, among which prescription 1 （Galla Chinensis ＋ Fructus Mume） exhibited the strongest antibacterial effect.对引起半滑舌鳎腐皮病的病原进行了形态学观察、生理生化鉴定,结果为哈维氏弧菌,动物回归试验显示该菌对半滑舌鳎有较强的致病作用。为了探索其致病机理,采用PCR方法进行了外膜蛋白(OMP)基因检测,结果发现,3株代表菌均携带OMP基因。通过中草药药敏试验的结果显示:五倍子、乌梅、沙棘、苏木四种单味中药对哈维氏弧菌有较强的抑菌作用;陈皮、石菖蒲、鱼腥草、马齿苋、穿心莲、桉树叶、薄荷等对病原哈维氏弧菌无作用;根据中药组方法则拟定了三个组方,其中由五倍子和乌梅组成的组方抑菌效果最强。

关 键 词：Cynoglossus semilaevis Gunther Skin ulcer disease PATHOGEN Outer membrane protein Chinese herbal medicine 

分 类 号：S943[农业科学—水产养殖]