Screening of Preprocessing Method of Biolog for Soil Microbial Community Functional Diversity  

Biolog法测定土壤微生物群落功能多样性预处理方法的筛选（英文）

作　　者：党雯[1] 郜春花[2] 张强[2] 李建华[2] 卢朝东[2] 靳东升[2] 卢晋晶 

机构地区：[1]山西大学生物工程学院,山西太原030006 [2]山西省农业科学院农业环境与资源研究所,山西太原030006

出　　处：《Agricultural Science & Technology》2015年第10期2247-2251,2255,共6页农业科学与技术（英文版）

基　　金：Supported by National and International Scientific and Technological Cooperation Project"The application of Microbial Agents on Mining Reclamation and Ecological Recovery"(2011DFR31230);Key Project of Shanxi academy of Agricultural Science"The Research and Application of Bio-organic Fertilizer on Mining Reclamation and Soil Remediation"(2013zd12);Major Science and Technology Programs of Shanxi Province"Key Technology Research and Demonstration of mining waste land ecosystem Restoration and Reconstruction"(20121101009)~~

摘　　要：As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the preprocessing meth- ods reported in the literatures were not the same. In order to screen the best pre- processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B＇s overall trend of AWCD values was better than A and C＇s. Method B＇s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car- bon source utilization richness index of the two treatments were B〉C〉A, while the Mclntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smallest variance. Method B＇s principal component analysis was better than A and C＇s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃ was the best one, because it was simple, convenient, and with good repeatability.作为测定微生物群落功能多样性主要方法之一的biolog法,由于操作简单、灵敏度高、分辨力强、获得数据量丰富,受到众多研究者的青睐,但文献中报道的有关实验预处理方法却不尽相同。为了筛选最佳的预处理方法,通过比较文献中3种较为常用的方法,研究不同预处理对biolog法测定微生物群落功能多样性的影响。结果表明:AWCD值总体变化趋势B方法优于A、C;微生物对六类碳源的利用率中B方法对大部分碳源利用较高,且结果较稳定;两种处理的Simpson指数、Shannon丰富度指数及碳源利用丰富度指数均B>C>A,而Mc Intosh指数、Shannon均匀度结果不太稳定,但经方差分析,其差异不显著,且B方法的方差最小;主成分分析的结果 B方法优于A与C。总之,在250 r/min的转速下震荡30 min,并于28℃条件下培养接种平板的处理(B法)效果较好,其测定结果重现性好,且操作简便快捷,是比较理想的预处理方法。

关 键 词：Biolog method Preprocessing method Soil microbial community Func- tional diversity AWCD values 

分 类 号：S154.3[农业科学—土壤学]