An Orthogonal Experiment of Papain-Assisted Extraction of Polysaccharides from Agrocybe aegirit  

木瓜蛋白酶提取茶树菇多糖的相关因素的正交试验研究（英文）

作　　者：齐小琼[1] 陈丽[1] 

机构地区：[1]湖北第二师范学院化学与生命科学学院/植物抗癌活性物质提纯与应用湖北省重点实验室,湖北武汉430205

出　　处：《Agricultural Science & Technology》2015年第10期2331-2333,2336,共4页农业科学与技术（英文版）

基　　金：Supported by Youth Fund of National Natural Science Foundation of China(31201242/C130405);Science Research Program of Education Department of Hubei Province(Q20143004)~~

摘　　要：[Objective] This study aimed to explore optimum conditions for papain-as- sisted extraction of polysaccharides from Agrocybe aegirit. [Method] Because enzyme is capable of destroying organizational structure of cells, papain was selected to cat- alyze cells to let polysaccharides out. An orthogonal experiment was carried out to investigate the effects of hydrolysis temperature, hydrolysis time, enzyme concentra- tion and solid to liquid ratio on extraction of polysaccharides from A. aegirit. [Result] Through the orthogonal experiment, the optimum papain-assisted extraction conditions were obtained： hydrolysis temperature, 45 ℃; hydrolysis time, 2 h; enzyme concen- tration 2%; solid to liquid ratio, 1：80. [Conclusion] This study will provide a reference for extraction of polysaccharides from A. aegirit.[目的]探索木瓜蛋白酶提取茶树菇多糖的最佳反应条件。[方法]利用酶能够破坏细胞的组织结构的原理,选用木瓜蛋白酶酶解细胞获取多糖。同时考虑到影响酶作用的几个因素,利用正交法研究了木瓜蛋白酶在4个因素(酶解温度、酶解时间、酶浓度和料液比)3水平下的提取效果。[结果]正交试验确定了木瓜蛋白酶提取茶树菇多糖的得率最高的是:酶解温度为45℃、酶解时间2 h、酶解浓度为2%、料液比1∶80,在该提取条件下,茶树菇多糖的得率为5.5%。[结论]正交试验结果确定了木瓜蛋白酶法提取茶树菇多糖的最佳反应条件。

关 键 词：Agrocybe aegirit   Papain Orthogonal experiment Polysaccharide extraction 

分 类 号：S646[农业科学—蔬菜学]