富含碳水化合物微藻的筛选、鉴定及其在不同氮浓度条件下的产糖分析  

Screening and Identification of Carbohydrate-rich Microalgae and Carbohydrate Production under Different Concentrations of Nitrogen

作　　者：肖仕圆 许敬亮[1] 袁涛[1,2] 赵月[1,2] 吴浩[1] 李谢昆[1,2] 袁振宏[1] 

机构地区：[1]中国科学院广州能源研究所中国科学院可再生能源重点实验室,广州510640 [2]中国科学院大学,北京100049

出　　处：《新能源进展》2015年第5期340-345,共6页Advances in New and Renewable Energy

基　　金：国家高技术研究发展计划(863计划;2013AA065803);国家自然科学基金(2117623;21211140237);广东省科技攻关项目(2013B010403021);广州市科技攻关项目(2013J4300026)

摘　　要：以实验室保存的4株藻株为研究对象,分析了它们的生长速率和淀粉等糖类的含量,其中1株富含碳水化合物的微藻#3,其生长速率和淀粉含量分别可达0.086 g/(L·d)和225.2 mg/L。18S r DNA聚类分析表明,其属于栅藻科链带藻属Desmodesmus sp。缺N条件下,微藻生长速率慢,总糖积累快;而在其他不同N浓度条件下,生长速率趋势一致,总糖积累速度不尽相同。The growth rate and starch content of four strains of microalgae preserved in our laboratory were analyzed. A carbohydrate-rich microalgae #3, as one of the four, was screened. Its growth rate and starch content could attain to 0.086 g/（L·d） and 225.2 mg/L, respectively. Further, microalgae #3 was identified as Desmodesmus sp. based on 18 s r DNA cluster analysis. The growth rate of Desmodesmus sp. #3 was low and the total sugar accumulation was fast when cultured without adding nitrogen. The growth rate change at different nitrogen concentration was almost similar, but total sugar accumulation speed presented different change tendency.

关 键 词：微藻 18S RDNA 总糖 

分 类 号：TK6[动力工程及工程热物理—生物能]