野生花生全基因组抗病相关LOX基因的生物信息学分析  

Bioinformatics Analysis on LOX Genes Related to Disease Resistance in Whole Genome of Arachis duranensis and Arachis ipaёnsis

作　　者：宋辉[1] 赵术珍[1] 侯蕾[1] 乔善良 赵传志[1] 李爱芹[1] 王兴军[1] 

机构地区：[1]山东省农业科学院生物技术研究中心,山东济南250100 [2]鱼台县林业局,山东鱼台272300

出　　处：《山东农业科学》2015年第10期1-7,共7页Shandong Agricultural Sciences

基　　金："十二五"国家"863"计划项目(2013AA102602);山东省生物资源创新项目

摘　　要：脂肪氧合酶（lipoxygenases，LOX）是生物体内一种重要的双加氧酶，其在植物发育以及响应生物和非生物胁迫时发挥重要作用。本研究从 Arachis duranensis 和 Arachis ipaёnsis 两个野生花生基因组中各鉴定出18个 LOX 基因，分别命名为 AdLOX1～18和 AiLOX1～18。在 AdLOX5、AdLOX7、AiLOX8和 AiLOX18基因的CDS 序列中发现提前终止翻译的终止密码子，而 AiLOX9基因的 CDS 序列过短，推测这些基因可能是假基因。染色体定位结果显示，AdLOX 和 AiLOX 基因主要分布在2、3、6、8、9和10号染色体上，且其直系同源基因分布在两套染色体相同或相近的位置。通过分析 AdLOX 和 AiLOX 基因与已知功能 LOX 基因的亲缘关系发现，Ad-LOX6、AdLOX9、AdLOX11、AdLOX12、AdLOX17、AiLOX1、AiLOX4、AiLOX6、AiLOX16和 AiLOX17基因可能参与花生的抗病过程，启动子分析结果也支持这一结论。基因选择压力研究表明，AdLOX 和 AiLOX 基因在进化历程中受到纯化选择。Lipoxygenases are important dioxygenases in cellular organisms.LOX genes contribute to plant development,biotic and abiotic stress tolerance.Eighteen AdLOX genes,named AdLOX1 -18,were i-dentified from Arachis duranensis genome.Eighteen AiLOX genes,named AiLOX1 ~18,were identified from Arachis ipaёnsis genome.Among them,the coding sequences of AdLOX5,AdLOX7,AiLOX8 and AiLOX18 genes contained premature translational -termination codon,and the length of AiLOX9 gene was too short,it suggested that they were pseudogenes.The chromosomal location results showed that AdLOX and AiLOX genes mainly distributed in chromosome 2,3,6,8,9 and 1 0.The orthologous gene pairs located at the same or similar position in A.duranensis and A.ipaёnsis chromosomes,respectively.Functional study of LOX gene in other plant species and phylogenetic analysis indicated that AdLOX6,AdLOX9,AdLOX11,AdLOX12,Ad-LOX17,AiLOX1,AiLOX4,AiLOX6,AiLOX16 and AiLOX17 genes were possibly involved in plant disease re-sistance.Promoter analysis results also testified that.The gene selection pressure results indicated that AdLOX＆amp;nbsp;and AiLOX genes experienced purify selection during the evolutionary process.

关 键 词：野生花生 LOX 基因家族 抗病 系统发育 选择压力 

分 类 号：S656.201[农业科学—果树学]