二甲双胍对2型糖尿病大鼠内脏脂肪组织visfatin的作用和机制研究  

Effects of metformin on serum visfatin and visfatin expression in visceral adipose tissue of type 2 diabetic rats

作　　者：李健[1] 王怀国[2] 王坤[1] 王文汇[3] 黄秉统[1] 

机构地区：[1] 聊城市人民医院内分泌科,山东省聊城252000 [2] 聊城市人民医院肾内科,山东省聊城252000 [3] 山东大学齐鲁医院(青岛)内分泌科

出　　处：《中国医师杂志》2015年第10期1528-1531,共4页Journal of Chinese Physician

摘　　要：目的 探讨二甲双胍对2型糖尿病大鼠血清及内脏脂肪组织中visfatin表达水平的影响.方法 40只雄性Wistar大鼠按随机数字表法分为正常对照组（NC）和糖尿病组,用高糖高脂饮食加小剂量链脲佐菌素建立2型糖尿病大鼠模型,成模大鼠按随机数字表法分为糖尿病对照组（DC）、二甲双胍治疗组（MET）和胰岛素治疗组（INS）,分别给予生理盐水、二甲双胍、甘精胰岛素治疗8周,检测各组大鼠用药前后体质量、内脏脂肪含量、生化指标,计算胰岛素抵抗指数（HOMA-IR）、大鼠肥胖评定指数（Lee index）,测定血清visfatin水平,实时定量PCR法检测内脏脂肪组织visfatin mRNA的水平.结果 与DC组比较,INS组血清visfatin及内脏脂肪组织visfatin mRNA水平未显著下降（P ＞0.05）;MET组血清visfatin及内脏脂肪组织visfatin mRNA水平显著降低（P＜0.01）,但仍高于NC组（P＜0.05）.结论 二甲双胍可降低2型糖尿病大鼠血清visfatin水平和内脏脂肪组织visfatin mRNA的表达,改善胰岛素抵抗.Objective To investigate the effects of metformin on serum visfatin and the level of visfatin mRNA in visceral adipose tissue of type 2 diabetic rats.Methods Forty Wistar rats were randomly assigned into normal diet group （NC） and diabetic group.The rats in the diabetic group were fed with high glucose and high fat diet, and then injected with streptozotocin （STZ）.The diabetic rats were divided into diabetic control （DC）, metformin （MET）, and insulin-treatment （INS） groups.Eight weeks later, body weight （BW） , visceral adipose tissue weight, and biochemical indicators were assessed.Homeostasis model assessment of insulin resistance （HOMA-IR） and Lee index were calculated.The serum visfatin levels were detected by enzyme-linked immunoassay （EIA）, and the visfatin mRNA levels of visceral adipose tissues were detected by real time polymerase chain reaction （PCR）.Results Compared to group DC, the visfatin levels of serum and visceral adipose tissue mRNA were lower in INS group, but did not have significant difference （P 〉 0.05）;the visfatin levels of serum and visceral adipose tissue mRNA in MET group were significantly lower （P 〈 0.01）.Conclusions Metformin can reduce the visfatin levels of serum and visceral adipose tissue mRNA, and improve the insulin resistance in type 2 diabetic rats.

关 键 词：二甲双胍/药理学 糖尿病 2型/药物疗法/代谢 烟酰胺磷酸核糖基转移酶/代谢 脂肪组织/代谢 

分 类 号：R587.1[医药卫生—内分泌]