大鼠急性肺损伤模型超声成像与病理的相关性研究  

Study on the correlation between ultrasound imaging and pathology in acute lung injury of rats

作　　者：赵伟英[1] 夏国园[2] 董梁[1] 孙建刚[2] 刘庆猛[3] 沈雪艳[1] 

机构地区：[1]绍兴文理学院医学院,浙江绍兴312000 [2]绍兴文理学院附属医院超声科 [3]绍兴第二医院病理科

出　　处：《中华超声影像学杂志》2015年第10期898-901,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：绍兴市科技局计划项目（21）14870057）;绍兴文理学院医学院人才基金项目（2014-2）

摘　　要：目的探讨大鼠急性肺损伤声像图与组织病理严重程度的相关性。方法20只雄性Wistar大鼠随机分为对照组（4只）和实验组（16只），用油酸和脂多糖分两个阶段诱导建立大鼠急性肺损伤模型，分别于两个阶段造模后行肺部超声检查，进行声像图量化评分；处死大鼠留取肺标本进行组织病理观察并评分，显微镜（200倍）下行肺泡计数。结果随着肺损伤的加重，肺声像图表现为胸膜线显示不清或消失、昏线密集分布、肺实变伴支气管充气征等改变。对照组声像图评分为（0．25±0．50）分，肺损伤病理评分为（0．50±0．58）分，肺泡数（25±3）个；油酸组则分别为（2．86±1．35）分，（6．28±0．76）分，（10±2）个；油酸＋脂多糖组则分别为（5．83±2．32）分，（9．83±0．98）分，（6±2）个。声像图评分、病理评分与肺泡计数在三组间比较差异均有统计学意义（P〈0．01）。结论大鼠急性肺损伤声像图与组织病理严重程度呈正相关，声像图能有效地评价大鼠急性肺损伤严重度。Objective To evaluate the correlation between ultrasonography and histopathology in acute lung injury of rats. Methods Twenty male Wistar rats were randomly divided into control group （4 rats） and experimental group （16 rats）,in the experimental group acute lung injury models were step-by- step induced by oleic acid （OA） and sequentially by OA and lipopolysaccharide （LPS）. After the end of each step of models,ultrasound examination was applied,and the rats were sacrificed to take the lung specimens to be observed. The ultrasonography and pathological results were analyzed, and the numbers of alveolar were counted under microscope （200 times）. Results With the aggravation of acute lung injury, the sonographic findings showed unclear or disappeared pleural line, close-set of B-lines, and pulmonary consolidation with air bronchogram. For control group, the ultrasonographic score was 0.25 ± 0.50, lung injury pathological score was 0.50 ± 0.58, and the numbers of alveolar were 25 ± 3. For OA group, the ultrasonographic score was 2.86± 1.35, lung injury pathological score was 6.28 ± 0.76, and the numbers of alveolar were l0± 2. For OA ＋ LPS group, the ultrasonographic score was 5.83 ± 2.32, lung injury pathological score was 9.83 ± 0.98,and the numbers of alveolar were 6 ± 2. All 3 groups were significant different on ultrasonographic score, pathological score and alveolar counts （P 〈 0.01）. Conclusions The ultrasonography image of acute lung injury of rats were positively correlated with its histopathologieal alteration.The ultrasonography can effectively evaluate the degree of acute lung injury of rats.

关 键 词：超声检查 急性肺损伤 

分 类 号：R563.8[医药卫生—呼吸系统] R445.1[医药卫生—内科学] R-332[医药卫生—临床医学]