天麻素磷脂复合物的制备工艺考察  

Investigation of Preparation Technology of Gastrodin Phospholipid Complex

作　　者：王蓉[1] 胡容峰[1] 丁领振[1] 黄丽丹[2] 王斌[1] 方文悠[1] 

机构地区：[1]安徽中医药大学研究生部,省部共建新安医学教育部重点实验室,安徽省中药研究与开发重点实验室,安徽省“115”新安医药研究与开发创新团队,合肥230038 [2]安徽省食品药品检验所,合肥230051

出　　处：《中国实验方剂学杂志》2015年第21期14-16,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274100);国家科技支撑计划项目(2012BAI26B03);安徽省高层次人才创新创业基金项目(2009Z061);安徽省学术和技术带头人及后备人选科研活动经费项目(皖人社秘2011-381号-26)

摘　　要：目的:优选天麻素磷脂复合物的制备工艺,为该成分的开发应用提供参考。方法:采用溶剂挥发法制备天麻素磷脂复合物,以天麻素与大豆磷脂的结合率为指标,结合单因素试验和正交试验进行系统研究,考察了反应时间、温度、溶剂类型、药脂比、天麻素质量浓度等因素对磷脂复合物制备工艺的影响。结果:天麻素磷脂复合物的最佳工艺条件为以四氢呋喃为反应溶剂,反应温度40℃,反应时间0.5 h,天麻素与大豆磷脂的投料比1∶2,天麻素质量浓度20 g·L-1,转速100 r·min-1。复合物结合率98.3%。结论:建立的制备工艺稳定可行,可为天麻素的进一步开发利用提供条件。Objective： To optimize conditions for formation of gastrodin phospholipid complex.Method： The complex was prepared by solvent evaporation method. Taking binding rate of gastrodin and soybean phospholipid as index,effects of reaction solvent type,ratio of gastrodin to phospholipid,reactive temperature,reactive time and the concentration of reactant on preparation technology were studied by single factor tests and orthogonal design. Result： Optimum preparation conditions of gastrodin phospholipid complex were obtained as follows： tetrahydrofuran as reaction solvent, gastrodin-soybean phospholipid（ 1 ∶ 2）, reaction temperature at40 ℃,reaction time of 0. 5 h,the concentration of gastrodin 20 g·L- 1,speed of 100 r·min- 1. Binding rate of the complex was up to 98. 3%. Conclusion： Optimized preparation technology in this research is stable and feasible,it can provide conditions for further study of gastrodin.

关 键 词：天麻素 磷脂复合物 结合率 溶剂挥发法 

分 类 号：R283.6[医药卫生—中药学]