HPLC同时测定白及中3种成分的含量  

Simultaneous Determination of Three Components in Bletillae Rhizoma by HPLC

作　　者：张晓静[1] 赵艳霞[1] 邓雁如[1] 丁菲菲[1] 田华[1] 

机构地区：[1]天津中医药大学天津市中药化学与分析重点实验室,天津300193

出　　处：《中国实验方剂学杂志》2015年第21期40-42,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505302)

摘　　要：目的：建立HPLC同时测定白及中红果酸,gymnosideⅠ,militarine 3种成分的含量。方法：采用Waters Symmetry ShieldTMRP18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈（A）-0.05%磷酸水（B）梯度洗脱（0~10 min,5%~20%A;10~35min,20%~35%A;35~50 min,35%~100%A）,流速1.0 m L·min-1,检测波长225 nm,柱温25℃。结果：3种成分均能达到基线分离,线性关系良好,加样回收率在95%~105%。结论：该方法专属性强,灵敏度高、重复性好、简单易行,可用于同时测定白及药材中红果酸,gymnosideⅠ,militarine 3种成分的含量,可为白及药材的质量控制提供依据。Objective： To develop a HPLC method for simultaneous determination of（ 2R）-2-（ phydroxybenzyl） malic acid, gymnoside Ⅰ and militarine contents in Bletillae Rhizoma. Method： The chromatographic conditions were follows： Waters Symmetry ShieldTMRP18column（ 4. 6 mm × 250 mm,5 μm）,acetonitrile（ A）-0. 05% phosphoric acid water（ B） as mobile phases for gradient elution（ 0-10 min,5%-20%A; 10-35 min,20%-35% A; 35-50 min,35%-100% A）. The flow rate was of 1. 0 m L·min- 1. The detection wavelength was set at 225 nm and the column temperature was 25 ℃. Result： All of the three components achieved baseline separation and showed good linearity. The average recoveries were between 95%-105%.Conclusion： This is a specific,sensitive,repeatable and simple method for simultaneous determination of（ 2R）-2-（ p-hydroxybenzyl） malic acid,gymnosideⅠand militarine contents in Bletillae Rhizoma. This method can provide basis for the quality control of Bletillae Rhizoma.

关 键 词：红果酸 1-(4-β-D-吡喃葡萄糖氧苄基-(2R)-2-异丁基苹果酸 1 4-二[4-(β-D-吡喃葡萄糖氧)苄基]-2-异丁基苹果酸酯 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]