黄芪甲苷对阻塞性肺疾病大鼠caspase-12表达影响  

Effect of Astragalus saponins on caspase-12 expression in rats with chronic obstructive pulmonary disease

作　　者：楚琪[1] 李昭[1] 汲坤[1] 郭庆革 

机构地区：[1]沈阳医学院,辽宁沈阳110034 [2]沈阳军区政治部军事监狱卫生所

出　　处：《中国公共卫生》2015年第11期1406-1409,共4页Chinese Journal of Public Health

基　　金：沈阳市科技局项目(F13-220-9-58)

摘　　要：目的观察黄芪甲苷对慢性阻塞性肺疾病(COPD)大鼠内质网应激诱导的肺泡上皮细胞凋亡的保护作用。方法将60只SD大鼠,随机分为对照组、模型组和黄芪甲苷低、中、高剂量组,每组12只;除对照组其余各组均采用气管内滴注脂多糖联合熏烟方法制备COPD大鼠模型,采用原位末端脱氧核苷酸转移酶标记法(TUNEL)法测定各组大鼠肺组织中细胞凋亡情况,采用Western blot法检测各组大鼠肺组织中caspase-3、caspase-12的表达水平。结果与对照组比较,模型组大鼠肺组织中细胞凋亡数量[(173.13±19.20)个/视野]明显升高;与模型组比较,黄芪甲苷高、中、低剂量组大鼠肺组织中细胞凋亡数量[分别为(149.23±8.95)、(118.77±9.47)、(78.87±9.59)个/视野]均减少(P<0.01);与对照组比较,模型组大鼠肺组织中caspase-3、caspase-12表达[分别为(0.847±0.103)、(0.706±0.042)]均升高;与模型组比较,黄芪甲苷高、中、低剂量组大鼠肺组织中caspase-3表达量[分别为(0.412±0.057)、(0.508±0.084)、(0.714±0.061)]与caspase-12表达量[分别为(0.341±0.052)、(0.393±0.065)、(0.516±0.087)]均降低(P<0.01)。结论黄芪甲苷可减轻内质网应激介导的肺组织细胞凋亡,其机制可能与降低大鼠肺组织中凋亡相关蛋白caspase-3、caspase-12表达有关。Objective To investigate the protective effect of Astragalus saponins methyl glycoside on the apoptosis of alveolar epithelial cells induced by endoplasmic reticulum stress in rats with chronic obstructive pulmonary disease （COPD）. Methods Sixty Sprague-Dawley（SD）rats were divided into a control group, a model control group, and low-, medium- and high-dose Astragalus saponins methyl glycoside groups randomly（ 12 rats in each group）. Except for those in control group, all rats were exposed to cigarette smoke once a day continuously for 28 days following intratracheal instillation of lipopolysaceharide（ LPS ）at the first and 14th day of the treatment to establish COPD model. The apoptosis of alveolar epithelial cells was analyzed using terminal-deoxvnucleoitidvl transferase mediated nick end labeling （TUNEL）. The aspartic acid specific cysteine proteinase 3 （caspase-3）and aspartic acid specific cysteine protease-12 （caspase-12）in lung tissues of the rats were detected using Western blot. Results Compared with that of the control group,the number of apoptotic cells in the model group increased significantly（ 173.13 ± 19. 20 vs. 0. 83 ±0. 34 per mi- croscopic field,P 〈 0. 01 ） ;while compared to that of the model control group, the number of apoptotic cells decreased significantly in high-, medium-, and low-dose Astragalus saponins methyl glycoside groups （ 149. 23 ± 8. 95,118. 77 ± 9. 47 ,and 78.87 ± 9. 59 per microscopic field） （ all P 〈 0. 01 ）. The expressions of caspase-3 （0. 847 ± 0. 103 ） and caspase-12 （0. 706 ± 0. 042） in the lung tissue of the rats of model control group increased significantly compared to that of the rats of the control group（ all P 〈 0. 01 ） ;whereas compared to those of model control group,the expressions of caspase-3 （ 0.412 ± 0. 057,0. 508 ± 0. 084, and 0. 714 ± 0.061 ） and caspase- 12 （ 0. 341 ± 0. 052,0. 393 ± 0. 065, and 0. 516 ± 0. 087 ） decreased significantly in the rats of high-, medium-, and low-dos

关 键 词：慢性阻塞性肺疾病(COPD) 黄芪甲苷 凋亡 CASPASE-3 CASPASE-12 

分 类 号：R181.24[医药卫生—流行病学]