含萘直链多胺化合物的荧光pH效应及核苷磷酸盐的分子识别  

The pH Effect on the Fluorescence of Linear Poly-amino-compounds and the Recognition of Nucleoside Phosphates

作　　者：梅明华[1] 阎晶晶[1] 吴世康[1] 

机构地区：[1]中国科学院理化技术研究所,北京100101

出　　处：《物理化学学报》2002年第8期686-691,共6页Acta Physico-Chimica Sinica

基　　金：国家重点基础研究发展规划(G2000078100)资助项目

摘　　要：合成了三种含萘但链长不同的多胺类化合物.研究了它们在不同pH条件下的荧光光谱,发现所研究化合物的荧光强度与pH值间有着强烈的依赖关系,因此这类化合物可用作测定溶液pH值的荧光探针.在上述化合物溶液中分别引入不同的核苷磷酸盐(ATP、ADP、AMP),发现所研究的化合物如N-(2-氨乙基)-N'-(2-犤(1-萘甲基)氨乙基犦)乙二胺(化合物3),其荧光可被不同的核苷磷酸盐所猝灭.以Stern-Volmer常数(KSV)或猝灭常数(Kq)作为评估猝灭能力大小的标准表明,不同核苷磷酸盐的猝灭能力是各不相同的.其中ATP有着对化合物3荧光最大的猝灭功能.实验结果表明,分子尺寸的匹配和化合物中基团的空间合理排布是化合物和核苷磷酸盐间能发生相互作用的重要根据,而猝灭则强烈地依赖于这类物种间的相互作用,因此可利用化合物荧光强度的改变来识别各种不同的核苷磷酸盐.Three kinds of naphthalene containing poly-amino-compounds with different chain length have been synthesized in this work. Their fluorescence spectra were recorded under different pH conditions and showed that the fluorescence intensity of studied compounds depends on the pH value very strongly. It means that this kind of compounds can be used as a probe for the determination of pH value of solution. In the solution of these compounds, different kinds of nucleoside phosphate (ATP, ADP and AMP) were introduced respectively. It was found that the fluorescence of compounds studied in this work such as C10H7CH2(NHCH2CH2)(3)NH2 (compound 3) can be quenched by different nucleoside phosphate and their quenching ability which was evaluated by the Stern-Volmer constants or quenching constants were also different. The results obtained showed that the ATP has the strongest ability to the fluorescence quenching of compound 3. It demonstrated that the molecular size matching and the sterically arrangement of groups of studied compounds with nucleoside phosphates are very important for the interaction of them. And the quenching ability depends on the interaction of these species strongly. Evidently, this phenomenon can be used as a method for the molecular recognition of nucleoside phosphate by the changing of fluorescence intensity of these compounds.

关 键 词：萘 荧光pH效应 分子识别 荧光化学敏感器 核苷磷酸盐 多胺类化合物 分子相互作用 阳离子识别 

分 类 号：O657.3[理学—分析化学]