第5天冷冻囊胚解冻培养4h与过夜培养20h移植的临床结局比较  

Comparison of the pregnancy outcomes of frozen blastocyst transferred 4 hours after being thawed or 20 hours after being cultured overnight

作　　者：习海涛[1] 葛红山[1] 池海虹[1] 陈华[1] 朱春芳[1] 张帆[1] 吕杰强[1] 

机构地区：[1]温州医科大学附属第二医院生殖中心,浙江温州325027

出　　处：《温州医科大学学报》2015年第10期762-765,共4页Journal of Wenzhou Medical University

基　　金：温州市科技局科研基金资助项目(Y20120136)

摘　　要：目的:通过比较第5天冷冻囊胚解冻培养4 h与过夜培养20 h后移植的临床结局,探讨囊胚解冻后到移植用以培养的适宜时间。方法:在第5天冷冻囊胚解冻移植周期中,按照囊胚解冻后到移植用以培养的时间不同分为2组:解冻4 h后移植组(A组,移植周期157个,囊胚268枚)和提前解冻过夜培养20 h后移植组(B组,移植周期227个,囊胚370枚),比较其妊娠结局。结果:B组的囊胚孵化率明显高于A组,差异有统计学意义(P<0.01);B组周期取消率稍高于A组,但差异无统计学意义(P>0.05);B组在平均移植胚胎个数上稍少于A组,但差异无统计学意义(P>0.05);A、B 2组在临床妊娠率、胚胎种植率、流产率和多胎妊娠率上差异无统计学意义(P>0.05)。结论:囊胚提前解冻过夜培养20 h与培养4 h后移植的临床结局无明显差异,操作人员对于囊胚解冻时间上将有更多选择。Objective： To compare the pregnancy outcomes when frozen blastocyst was transferred 4 hours after being thawed or 20 hours after being cultured overnight. Methods： For the 5 days freezing thawing blastocysts transplantation cycle, all the cycles are divided into two groups based on different culture time before transfer, those blastocysts transferred 4 hours after being thawed were labeled as A group（n=157） and those cultured overnight before being transferred the next day were included in B group（n=227）. The pregnancy outcomes were compared between the two groups. Results： The proportion of thawing cycles leading to no transfer did not differ between groups（1.2% versus 2.2% for A and B, respectively）. However, the proportion of hatched blastocysts derived from B group was significantly higher than that from A group（41.7% versus 86.5%, respectively, P〈0.001）. All differences of the clinical pregnancy rate, implantation rate, miscarriage rate and multiple pregnancy rate between two groups have not yet reached the statistical significance. Conclusion： Overnight culture of thawed blastocyst before transfer does not affect the main pregnancy outcomes. For blastocyst thawing time, operators will have more choices.

关 键 词：囊胚 玻璃化冷冻 解冻 过夜培养 移植 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]