大鼠脊髓损伤程度与血清β-淀粉样前体蛋白及超敏C反应蛋白的相关性研究  

Relation between the degree of the acute spinal cord injury and the serum β-amyloid precursor protein and hypersensitive-cross-reactive protein levels

作　　者：季英楠[1] 阿良[1] 赵忠海[1] 邓纯博[1] 

机构地区：[1]沈阳医学院附属中心医院骨外二科,辽宁沈阳110000

出　　处：《大连医科大学学报》2015年第5期425-428,共4页Journal of Dalian Medical University

基　　金：沈阳医学院科技基金项目(20142035)

摘　　要：目的探讨大鼠急性脊髓损伤(SCI)程度与血清β-淀粉样前体蛋白(β-APP)和超敏C反应蛋白(hs-CRP)含量变化的相关性。方法取雌性SD大鼠90只,采用动脉夹压力法制备大鼠脊髓轻度损伤(轻度损伤组,30只)和重度损伤(重度损伤组,30只)动物模型,另30只大鼠仅行椎板切除作为假手术组。通过行为学观察(BBB评分)了解脊髓损伤程度及变化,分别于造模成功后12 h、24 h、3 d、7 d、14 d采股静脉血,ELISA检测不同程度SCI大鼠伤后血清β-APP、hs-CRP含量变化。结果重度脊髓损伤组与轻度损伤、假手术组各时间点BBB评分差异有显著性意义(P<0.05)。β-APP于损伤后24 h达到高峰后逐渐回落,hs-CRP于3 d达到高峰后回落。重度脊髓损伤组与轻度脊髓损伤、假手术组各时间点β-APP、hs-CRP比较差异均有显著性意义(P<0.05);轻度脊髓损伤组与假手术组β-APP、hs-CRP水平除14 d点差异无显著性意义外,其余各时间点差异均有显著性意义(P<0.05)。结论急性SCI大鼠血清β-APP、hs-CRP水平变化可反映SCI的严重程度,β-APP比hsCRP的变化敏感。Objective To investigate the relation to the degree of the acute spinal cord injury （ SCI） by detecting the serumβ-amyloid precursor protein （β-APP） and hypersensitive-cross-reactive protein （ hs-CRP） levels after acute spinal cord injury in rate at different time points.Methods Ninety healthy adult female SD rats were randomly assigned into 3 groups.The bulldog clamp was used to create models of severe SCI （ group A） and mild SCI （ group B） .In group C, only laminectomy was performed without SCI.The ethological study （ BBB scores） was used to learn the degree of the acute spi-nal cord injury.The serum was collected at 12 h, 24 h, 3 d, 7 d, and 14 d after operation through femoral vein.Serumβ-APP and hs-CRP were detected by ELISA.Results The serumβ-APP and hs-CRP reached the peak at 24 h and 3 d, respectively after acute SCI.Serumβ-APP and hs-CRP levels in SCI groups （ A and B） were both significantly higher than those in without SCI group （ C） at all time points （ P〈0.05） , except at 14 d between group B and group C. Conclusions The serumβ-APP and hs-CRP levels are positively associated with the severity of SCI.β-APP would be a more sensitive indicator than hs-CRP in a certain time.A acute SCI model of SD rats can be established by bulldog clamp, in order to mimic the clinical SCI.

关 键 词：脊髓损伤 血清β-淀粉样前体蛋白 超敏C反应蛋白 

分 类 号：R651.2[医药卫生—外科学]