东海陆架表层沉积物细菌群落结构及地理分布研究  

COMMUNITY STRUCTURE AND GEOGRAPHICAL DISTRIBUTION OF BACTERIAL ON SURFACE LAYER SEDIMENTS IN THE EAST CHINA SEA

作　　者：刘明华[1] 王健鑫[1] 俞凯成[1] 蒋然[1] 刘晓辉[1] 王帅兵[1] 刘雪珠[1] 

机构地区：[1]浙江海洋学院海洋微生物生态与应用实验室,舟山316022

出　　处：《海洋与湖沼》2015年第5期1119-1131,共13页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金面上项目;31270160号;J1310037号;浙江省自然科学基金项目;LY12C03003号

摘　　要：本研究通过构建16S r DNA克隆文库,对东海陆架五个站点的沉积物样品进行群落结构及地理分布研究。结果表明:东海陆架沉积物细菌分属于13个类群,包括变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteria)、硝化螺旋菌门(Nitrospira)、WS3、拟杆菌门(Bacteroidetes)、螺旋体门(Spirochaetes)、OP8、浮霉菌门(Planctomycetacia)、疣微菌门(Verrucomicrobiae)和芽单胞菌门(Gemmatimonadetes),其中变形菌门(γ-变形菌纲、δ-变形菌纲)和绿弯菌门为优势菌群,Latescibacteria(WS3)、疣微菌门和芽单胞菌门分别是站点DH6、DH21和DH9的特有类群。五个站点细菌多样性从高到低排序依次为DH9>DH21>DH17>DH6>DH16,其群落结构和丰度与各站点不同沉积环境类型有关。We explored bacterial community structure and geographical distribution on the top layer sediments in five sites in the East China Sea by constructing 16 S r DNA clone library. The results show that the bacteria were belonged to 13 groups including Proteobacteria, Firmicutes, Acidobacteria, Chloroflexi, Actinobacteria, Nitrospira, WS3, Bacteroidetes, Spirochaetes, OP8, Planctomycetacia, Verrucomicrobiae, and Gemmatimonadetes. The Proteobacteria and Chloroflexi were the dominant groups of all sites. WS3, Verrucomicrobiae, and Gemmatimonadetes were unique to Sites DH6, DH9, and DH21, respectively. The descendent order of bacterial diversity in site is DH9 DH21 DH17 DH6 DH16. In addition, community structure and abundance were closely related to different types of local sedimentary environments.

关 键 词：细菌多样性 群落结构 16S RDNA 沉积物 

分 类 号：Q933[生物学—微生物学]