水杨酸和茉莉酸甲酯对高温龙须菜(Gracilariopsis lemaneiformis)理化及基因表达的影响  

EFFECTS OF SALICYLIC ACID AND METHYL JASMONATE ON PHYSICOCHEMICAL PROPERTY AND GENE EXPRESSION OF GRACILARIOPSIS LEMANEIFORMIS UNDER HEAT STRESS

作　　者：王重彬[1,2] 邹同雷 孙雪[1,2] 汪芳俊[1,2] 徐年军[1,2] 

机构地区：[1]宁波大学海洋学院,宁波315211 [2]浙江省海洋生物工程重点实验室,宁波315211

出　　处：《海洋与湖沼》2015年第5期1132-1138,共7页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目;31072229号;41376151号;宁波大学学科项目;xkl141053号

摘　　要：本文研究了两种抗逆植物激素——水杨酸(SA)和茉莉酸甲酯(MJ)对高温胁迫龙须菜的生长、脯氨酸和丙二醛(MDA)含量、超氧化物歧化酶(SOD)、碳酸酐酶(CA)、硝酸还原酶(NR)活性及4种相关基因表达的影响。结果表明,100μmol/L SA(SA100)、50μmol/L MJ(MJ50)、50μmol/L SA+25μmol/L MJ(SA50/MJ25)实验组对龙须菜生长促进作用较大,其相对生长速率分别为对照组的1.78、1.65和1.29倍。这3个处理组均可提高龙须菜脯氨酸含量,其中SA100作用最强;对MDA含量的降低程度以SA50/MJ25组最强。SA100增强SOD和CA活性的作用最大,而MJ50可增强NR活性。SA100处理HSP70、Mn SOD、CA和NR基因表达量分别为对照组的2.04、4.65、2.15和1.58倍,MJ50处理后Mn SOD、CA和NR基因表达量则分别降为对照组的0.47、0.50和0.46倍,而SA50/MJ25则促进了HSP70基因表达,降低了CA和NR基因表达。本研究表明SA和MJ可以缓解高温胁迫对龙须菜的不利影响,其中以SA100的作用最大,MJ50作用最小。Salicylic acid（SA） and methyl jasmonate（MJ） are two new types of phytohormone which can resist effectively in adverse environments. In this study, the effects of SA and MJ on growth, proline and MDA contents, activities of SOD, CA and NR, and 4 relevant gene expressions in high-temperature-cultured Gracilariopsis lemaneiformis, were investigated. Results showed that 100μmol/L SA（SA100）, 50μmol/L MJ（MJ50）, and 50μmol/L SA＋25μmol/L MJ（SA50/MJ25） experimental groups could promote notably the algal growth by 1.78, 1.65, and 1.29 times compared to the control, respectively. Proline contents were enhanced in all the three treatments, highest in SA100 group, whereas SA50/MJ25 had the strongest effect on reducing MDA content. In the SA100 group, enzymatic activities of SOD and CA were enhanced significantly, while enzymatic activity of NR in MJ50 group was improved. After treated with SA100, expressions of HSP70, Mn SOD, CA and NR genes were improved by 2.04, 4.65, 2.15, and 1.58 times of the control. In addition, expressions of Mn SOD, CA and NR genes in MJ50 group decreased by 0.47, 0.50, and 0.46 time of the control. In SA50/MJ25 group, expression of HSP70 gene was enhanced but those of CA and NR genes were depressed. Therefore, SA and MJ could alleviate heat stress on G. lemaneiformis strongest in SA100 group and smallest in MJ50 group.

关 键 词：龙须菜 水杨酸 茉莉酸甲酯 高温 基因表达 

分 类 号：Q946[生物学—植物学]