头低位卧床期间人体肠道益生菌多样性变化  

Observation of microbe diversity and dynamic changes of intestinal probiotic bacteria during head-down tilt bed rest

作　　者：雷浪伟[1] 韩炳星 兰海云[1] 张淑静[1] 陈斌[1] 

机构地区：[1]中国航天员科研训练中心航天医学基础与应用国家重点实验室航天营养与食品工程实验室,北京100094

出　　处：《中国微生态学杂志》2015年第11期1253-1257,共5页Chinese Journal of Microecology

基　　金：国家"863"计划"临床营养菌种资源库的建立及应用研究"(2014AA022209);总装实验技术课题"头低位卧床对人体肠道菌群的影响"(2011Y5409020)

摘　　要：目的观察了解头低位卧床期间人体肠道益生菌双歧杆菌属、乳酸杆菌属的数量及多样性的变化趋势。方法采集受试者-6°头低位连续卧床30d期间肠道便样,通过平板可培养技术、浓度梯度凝胶电泳技术和Ion Torrent基因组测序技术平台等实验方法,检测分析肠道粪便益生菌。结果卧床期间,可培养肠道菌群中的双歧杆菌、乳杆菌数量显著减少,益生菌中双歧杆菌出现不规律的缺失与恢复,肠道菌群多样性指数下降。结论头低位卧床期间肠道益生菌多样性发生了改变。益生菌数量减少,多样性指数下降,头低位卧床对肠道菌群有不利的影响。Objective To examine the microbial diversity and dynamic changes of intestinal microbe during head-down tilt bed rest（HDTB）.Methods The stool specimens of patients who were at a minus 6°headdown tilt besd rest for consecutive 30 days were collected;Intestinal probiotics were examined by using selective culture technique,Denaturing Gradient Gel Electrophoresis（DGGE）technique and the Ion Torrent personal genome machine system.Results The number of intestinal bacteria including Bifidobacteria and Lactobacilli decreased significantly,and the diversity of microbe descended during HDTB.Conclusion Intestinal microbial diversity changes during HDTB with the number of probiotic bacteria and diversity index decrease,suggesting an adverse effect of HDTB on intestinal microbe.

关 键 词：头低位卧床 肠道益生菌 多样性 

分 类 号：Q933[生物学—微生物学]