镉和萘单一及复合胁迫对红树植物白骨壤幼苗生理影响的差异分析  

Difference analysis on effects of single and combination stresses of Cd and Nap on physiology of mangrove Avicennia marina seedlings

作　　者：陆志强[1] 陈昌徐[2] 马丽[3] 章耕耘 郑文教[2] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]厦门大学环境与生态学院,福建厦门361102 [3]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《植物资源与环境学报》2015年第3期60-67,共8页Journal of Plant Resources and Environment

基　　金：福建省自然科学基金资助项目(2010J05098);国家海洋局海洋环境保护司2013年业务性科研课题(HD130701)

摘　　要：采用砂基培养法,对不同浓度镉(Cd)(0、25和150 mg·L-1)和萘(Nap)(0和10 mg·L-1)单一及复合胁迫45和90 d后红树植物白骨壤〔Avicennia marina(Forsk.)Vierh.〕的叶片叶绿素含量,根尖和叶片的MDA、As A和可溶性蛋白质含量及SOD和POD活性,根、茎、子叶和叶片中的Cd含量进行了比较分析。结果表明:胁迫处理45 d时,Cd-Nap复合胁迫处理组的叶片叶绿素a含量、根尖和叶片的可溶性蛋白质含量及SOD和POD活性大多显著高于相同浓度Cd单一胁迫处理组,叶片叶绿素b含量显著低于后者,Chla/Chlb值显著高于后者。胁迫处理90 d时,与相同浓度Cd单一胁迫处理组相比,Cd-Nap复合胁迫处理组叶片的叶绿素a、叶绿素b和总叶绿素含量以及Chla/Chlb值,根尖和叶片的MDA和As A含量总体上无显著差异,而根尖和叶片的可溶性蛋白质含量和POD活性及根尖的SOD活性均显著提高。经Cd-Nap复合胁迫处理后白骨壤幼苗的根、茎、子叶和叶片中的Cd含量总体上高于相同浓度Cd单一胁迫处理组,且随胁迫时间延长其差异越来越明显。研究结果显示:在Cd-Nap复合胁迫条件下Cd和Nap具有协同作用,随胁迫时间延长和Cd浓度提高,Cd-Nap复合胁迫对白骨壤幼苗的伤害逐渐增强,并能够促进各器官对Cd的累积。Chlorophyll content in leaf, MDA, AsA, soluble protein contents and SOD and POD activities in root tip and stem, Cd content in root, stem, cotyledon and leaf of mangrove Avicennia marina （Forsk.） Vierh. of different treatment groups after single and combination stressed for 45 and 90 d with different concentrations of cadmium （Cd） （including 0, 25 and 150 mg·L^-1） and naphthalene （Nap） （ including 0 and 10 mg ·L^-1 ） were comparatively analyzed by sand culture method. The results show that when stressed for 45 d, Chla content in leaf, soluble protein content and SOD and POD activities in root tip and leaf of Cd-Nap combination stress treatment group are mostly significantly higher than those of single stress treatment group with the same concentration of Cd, Chlb content in leaf is significantly lower than that of the latter, and Chla/Chlb ratio is significantly higher than that of the latter. When stressed for 90 d, compared with single stress treatment group with the same concentration of Cd, there are generally no significant differences in Chla, Chlb and total chlorophyll contents and Chla/Chlb ratio of leaf, MDA and AsA contents in root tip and leaf of Cd-Nap combination stress treatment group, while soluble protein content and POD activity in root tip and leaf and SOD activity in root tip are all increased significantly. After treated by Cd-Nap combination stress, Cd content in root, stem, cotyledon and leaf of A. marina seedlings is generally higher than that of single stress treatment group with the same concentration of Cd, and their differences are more and more obvious with prolonging of stress time. It is suggested that under condition of Cd-Nap combination stress, Cd and Nap have a synergistic effect, with prolonging of stress time and increasing of Cd concentration, damage of Cd-Nap combination stress to A. marina seedlings enhances gradually and Cd-Nap combination stress can promote the accumulation of Cd in different organs.

关 键 词：红树植物 白骨壤 镉 萘 复合胁迫 生理指标 

分 类 号：Q945.78[生物学—植物学] S793.9[农业科学—林木遗传育种]