川南地区维生素D受体Fok Ⅰ多态性与绝经后女性2型糖尿病的相关性  

The correlations between vitamin D receptor Fok Ⅰpolymorphism and type 2 diabetes in postmenopausal women

作　　者：王晓燕[1] 刘鑫[2] 余玥[2] 黄璨[2] 张珑山 刘洪明[2] 邓宇[3] 陈庄[1] 陈枫[1] 

机构地区：[1]泸州医学院附属医院医学实验中心,646000 [2]泸州医学院临床医学系,646000 [3]重庆医科大学临床医学系,400016

出　　处：《实用医学杂志》2015年第20期3427-3430,共4页The Journal of Practical Medicine

基　　金：四川省科技厅资助项目(编号:14JC01283-LH11);泸州市科技局资助项目(编号:2013LZLY-J04)

摘　　要：目的：探讨维生素D受体FokⅠ基因多态性与川南地区汉族绝经后女性2型糖尿病的相关性。方法：采用RFLP-PCR结合DNA测序方法检测糖尿病组160例和正常对照组190例 FokⅠ基因多态性。结果：糖尿病组和正常对照组FF、Ff和ff基因型频率分别为32．5％、47．5％、20％和15．8％、53．7％和30．5％，差异有显著性（P ＜0．05）。 F／f等位基因分布分别为56．3％、43．8％和42．6％、57．4％，差异有统计学意义（P ＜0．05）。FF基因型发生T2DM风险为Ff／ff基因型的2．568倍（95％ CI ＝1．246-5．292，P ＜0．05）。FF基因型2 h PG、HbA1C水平高于Ff／ff基因型（P ＜0．05）。结论：维生素D受体FokⅠ基因多态性与川南地区绝经后女性2型糖尿病的发生相关。Objective To study the correlations of VDR FokⅠgene polymorphism with type 2 diabetes in postmenopausal women of Han nationality in south Sichuan. Methods 160 patients with type 2 diabetes （T2DM） and 190 healthy cases were enrolled in the study. The VDR FokⅠgene polymorphisms were detected using RFLP-PCR and DNA sequencing. Results The FF, Ff and ff genotype frequencies were 32.5%, 47.5%and 20% in the T2DM group and 15.8%, 53.7%, 30.5% in the control group, respectively （P 〈 0.05）. The allele frequencies were 56.3%, 43.8% in the T2DM group and 42.6%, 57.4% in the control group, respectively （P 〈 0.05）. The risk of T2DM in the FF genotype people was 2.568 times higher than Ff/ff genotype （adjusted OR = 2.568, 95%CI = 1.246 - 5.292, P 〈 0.05）. The levels of 2 h PG and HbA1C in the FF genotype people were significantly higher than those of the Ff/ff genotype people （P〈0.05）. Conclusions There was an association between the VDR FokⅠgene polymorphism and type 2 diabetes incidence in the postmenopausal women in south Sichuan area.

关 键 词：糖尿病 2型 受体 骨化三醇 单核苷酸多态性 绝经后女性 Diabetes type 2 

分 类 号：R587.1[医药卫生—内分泌]