HPLC法测定人血清中拉莫三嗪浓度的方法改进  

Improvement of HPLC in Concentration Determination of Lamotrigine in Human Serum

作　　者：陈春枚[1] 赖剑锋[1] 陈志强[1] 

机构地区：[1]龙岩市第一医院药剂科,福建龙岩364000

出　　处：《中国药房》2015年第32期4504-4505,共2页China Pharmacy

摘　　要：目的：对测定人血清中拉莫三嗪（LTG）浓度的方法进行改进。方法：血清样品经甲醇处理后以高效液相色谱（HPLC）法进行测定,色谱柱为Hypersil BDS C18（200 mm×4.6 mm,5μm）,流动相为甲醇-KH2PO4（45∶55,V/V）,检测波长为250 nm,流速为1ml/min,柱温为35℃。结果：LTG血药浓度在0.5~30.0μg/ml范围内线性关系良好（r=0.999 9）,日内、日间RSD均〈5%,方法回收率为101.96%~102.74%。结论：改进后的方法简便、快速、准确,适合临床常规监测LTG血药浓度及LTG的药动学研究。OBJECTIVE： To establish a rapid method for the concentration determination of lamotrigine （LTG） in human se- rum. METHODS： HPLC was adopted after serum samples treated with methanol. The column was Hypersil BDS C18 （200 mm×4.6 mm,5μm） with the mobile phase of methanol-KH2PO4（45：55, V/V） at the flow rate of 1 ml/min, the detection wavelength was 250 nm, and the temperature was 35℃. RESULTS： The linear range of LTG was 0.5-30.0 μg/ml （r=0.999 9）. The RSDs of intra-day and inter-day were all less than 5% and the recovery was 101.96%-102.74%. CONCLUSIONS： The method is simple, accurate and fast, and suitable for the monitoring of LTG serum concentration.

关 键 词：拉莫三嗪 治疗药物监测 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学] R971.6[医药卫生—药学]