停乳链球菌GapC_(1-150aa)蛋白单克隆抗体的制备及其线性表位鉴定  

Preparation of monoclonal antibodies against Streptococcus dysgalactiae GapC_(1-150aa) and identification of a linear epitope to the MAbs

作　　者：张丽萌[1] 周雪[2] 魏玉华[1] 樊自尧[1] 汤炜[2] 代建 杨轩[2] 张建新[1] 杨汐静 刘道龙[2] 王成[2] 王健南[2] 于永忠[2] 吴志军[2] 于立权[2] 孙虎男[2] 马金柱[2] 宋佰芬[2] 朱战波[1] 崔玉东[1,2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]黑龙江八一农垦大学生命科技学院,黑龙江大庆163319

出　　处：《中国预防兽医学报》2015年第11期875-880,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然基金委课题(31072120);黑龙江省农垦总局课题(HNK11A-08-01-04);国家科技支撑现代奶业发展科技工程项目(2012BAD12B03;2012BAD12B05)

摘　　要：链球菌GapC蛋白是表达于各种链球菌的膜蛋白，具有良好的免疫原性。为研制预防链球菌感染的表位疫苗和研发以单克隆抗体（MAb）为基础的检测试剂盒，本研究利用表达的停乳链球菌GapC蛋白aa1～aa150片段制备了3株MAbs，并利用噬菌体展示技术对这3株MAbs的抗原表位进行分析，获得一个线性表位1377HDILDG142。该研究不仅有助于了解链球菌GapC的免疫保护作用机制，也有助于进一步研究表位疫苗和建立检测方法。GapC protein is a membrane protein expressed in all species of Streptococcus, which is one of the important antigens to induce antibody against the bacteria. To prepare the monoclonal antibody （MAb） and identify the epitopes of the GapC protein, three MAbs were prepared by fusion of the SP2/0 cells with the splenic cells from BALB/c mice immunized with the truncated recombinant GapC1-150aa protein of S.dysgalactiae expressed in E.coil. Furthermore, the antigen epitopes of the MAbs were screened using phage display panning, in which a linear epitope 137HDILDG 142 was identified. These results would facilitate study of the immunoprotective mechanisms of Streptococcus GapC, and development of epitope-based vaccines and MAb-based detection methods.

关 键 词：停乳链球菌 GAPC 单克隆抗体 B细胞抗原表位 

分 类 号：S852.61[农业科学—基础兽医学]