毛细管电泳法测定性保健品中的西地那非  

Determination of Sildenafil in Medicines for Erectile Dysfunction by Capillary Electrophoresis

作　　者：李润锴[1] 薄涛[1] 刘虎威[1] 李克安[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871

出　　处：《色谱》2002年第4期335-337,共3页Chinese Journal of Chromatography

摘　　要：用毛细管电泳法测定了万艾可片剂和两种市售性保健品中的西地那非。以 6 0mmol/L磷酸二氢钠溶液为缓冲溶液 (pH 5 0 ) ,在 35℃ ,30kV的条件下进行测定 ,结果西地那非的质量浓度为 0 0 7g/L～ 1 0 5 g/L时与相应峰面积的线性关系良好 (r =0 9985 ) ;在实际样品中的平均回收率为 97 4 % ;方法的重现性好 ,峰面积的RSD为4 7%。与目前通行的高效液相色谱法测定西地那非的结果相比 ,毛细管电泳方法所测得的结果同样准确可靠 ,而且样品处理简便 ,测试时间短 ,测试成本低 。A method has been developed for the determination of sildenafil in the medicines for erectile dysfunction by capillary electrophoresis. The samples were analyzed with 60 mmol/L NaH 2PO 4 (pH 5 0) running buffer at 35 ℃ capillary temperature and 30 kV voltage. A linear calibration was obtained from 0 07 g/L to 1 05 g/L of sildenafil ( r =0 998?5) with the RSD of 4 7% for peak area, and the average recovery was 97 4% The results were compared with those of HPLC, showing that the method is precise, simple and cost effective, and can be used as a complementary method to HPLC.

关 键 词：性保健品 高效液相色谱 毛细管电泳 西地那非 药物分析 

分 类 号：R977.12[医药卫生—药品] R917[医药卫生—药学]