针刺对视网膜变性大鼠感光细胞形态学变化的影响  

Effects of acupuncture on morphological changes of photoreceptor cells in rats with retinitis pigmentosa

作　　者：马瑞玲 吴根诚[2] 张仁 

机构地区：[1]北京京军医院针灸室,北京100690 [2]复旦大学上海医学院中西医结合系 [3]上海中医药科技服务中心

出　　处：《中国针灸》2015年第11期1149-1153,共5页Chinese Acupuncture & Moxibustion

基　　金：上海市博士后基金项目:05R214114

摘　　要：目的:观察N-甲基-N-亚硝脲(MNU)诱导的大鼠感光细胞的形态学变化以及针刺对其变化的影响。方法:16只SD大鼠以50mg/kg的剂量一次性腹腔注射MNU,诱导其视网膜变性,随机分为针刺组、模型组,各8只,另取4只SD大鼠作为对照组。针刺组选取"新明1"和"睛明"穴进行针刺治疗,留针30min,每天1次,治疗7d;模型组和对照组不进行针刺,饲养光照条件及抓取处理同针刺组。干预结束后2h断颈处死大鼠,观察各组大鼠视网膜视紫红质的分布、视杆末端以及视杆双极细胞的形态变化。结果:伴随着MNU诱导的大鼠视网膜感光细胞的丢失,模型组大鼠可见视紫红质被深度着色于残留的细胞体上,仅存零星的视杆末端以及少量残存的视杆双极细胞,外节、内节、胞体以及细胞的末端均有不同程度的影响;针刺组大鼠视紫红质的分布位置也发生变化,有较多层的感光细胞体,残存的视杆末端以及视杆双极细胞均较模型组多,全视网膜的损伤程度轻于模型组。结论:MNU对感光细胞形态学的损伤是全面的,针刺能够不同程度地抑制MNU诱导的大鼠视网膜感光细胞的形态学变化。Objective To observe the morphological changes of photoreceptor cells in rats with retinitis pigmentosa（RP）induced by N-methyl-N-Nitrosourea（MNU）and the effects of acupuncture against it.Methods A total of 16 SD rats were treated with one-time intraperitoneal injection of MNU（50mg/kg）to induce RP,and randomly divided into an acupuncture group and a model group,8rats in each one.In addition,4rats were selected as a control group.After model establishment,rats in the acupuncture group were treated with acupuncture at＂Xinming-1＂（Extra）and＂Jingming＂（BL 1）for 30 min,once a day for 7days;rats in the model group and control group received no treatment,and the feeding conditions and fixation were identical as the acupuncture group.2h after the end of intervention,rats were sacrificed by cervical dislocation to observe the morphological changes of rhodopsin,rod terminals and rod bipolar cells.Results Due to the loss of retina photoreceptor cells induced by MNU in rats,in the model group the rhodopsin was stained in residual cell bodies,and there were sporadic rod terminals and little rod bipolar cells;outer segments,inter segments,cell bodies and cell terminals were all affected at different levels.The distribution of rhodopsin was also changed in the acupuncture group,showing more bodies of photoreceptor cells,and the residual rod terminals and rod bipolar cells were more than those in the model group;the injury of retina was less than that in the model group.Conclusion MNU could lead to a comprehensive injury to the morphology of photoreceptor cells,however,acupuncture is capable of inhibiting morphological changes of photoreceptor cells induced by MNU.

关 键 词：视网膜变性 针刺 N-甲基-N-亚硝脲 感光细胞 形态学 

分 类 号：R246.8[医药卫生—针灸推拿学]