穴位的本态:穴位组织细胞化学的动态变化  

Entity of acupoint:kinetic changes of acupoints in histocytochemistry

作　　者：何伟[1] 吴美玲[1] 景向红[1] 白万柱[1] 朱兵[1] 喻晓春[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《中国针灸》2015年第11期1181-1186,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:81173205;81330087

摘　　要：采用免疫组织化学结合激光共聚焦技术,研究了正常穴位、不同部位穴位、刺激后的穴位、疾病状态的穴位局部组织细胞化学的动态变化,结果表明:1在正常的穴位处存在P物质(SP)、降钙素基因相关肽(CGRP)标记的神经纤维和形态完整的肥大细胞,这些肥大细胞同时也呈现出组织胺(HA)和五羟色胺(5-HT)的阳性标记。不同部位的穴位组织细胞化学成分相同但含量不同。2针刺大鼠"合谷"穴后,穴位局部表现为伤害性神经肽SP和CGRP在穴位局部表达增加,肥大细胞聚集脱颗粒,HA和5-HT表达增加。3在造成胃黏膜损伤后,体表敏化点的分布和内脏的神经节段分布类似,和经典穴位重叠率高。敏化穴位的局部也出现了和穴位刺激后相同的组织细胞化学改变。提示穴位处虽然没有一个特异性的解剖学结构,但是无论是疾病反应还是针刺刺激,其局部的组织细胞化学改变却是一致的。这可能就是穴位处组织细胞化学动态改变的基础。The present study aims to investigate the kinetic histocytochemical changes of acupoints in different condition.The expression of tryptase（＋）mast cells,histamine（HA）,serotonin（5-HT）and nociceptive neuropeptides including calcitonin gene-related peptide（CGRP）and substance P（SP）were observed by immunohistochemistry combined with confocal technology.Mast cells were labeled with anti-mast cell tryptase antibody and simultaneously with HA or 5-HT primary antibodies to observe their co-expression.The results showed that：1 SP and CGRP were expressed more highly on the cutaneous nerve fibers of＂Hegu＂（LI 4）after acupuncture stimulation than that of the control.Mast cells aggregated in close proximity to the blood vessels in intra-epidermis and dermis,and some of them with degranulation in the lower dermis and subcutaneous tissue of＂Hegu＂（LI 4）.Both mast cells and their granules appeared with HA（＋）and 5-HT（＋）expression at stimulated LI 4sites,while a few intact mast cells with a little expression of 5-HT and HA were distributed in areas of non-stimulated LI 4.2 The acupoints in different locations such as Baihui（GV 20）,Weishu（BL 21）,Zhongwan（CV 12）and LI 4had the same constituent but the contents were different.3 The histocytochemical responses of acupoints sensitized by the Gastric mucosa injury（GMI）were also investigated.GMI resulted in neurogenic plasma extravasation by Evans Blue（EB）in the skin of the acupoints over the back and abdomen,which mostly occurred in the T9-T11 dermatomere.The EB extravasation dots just like acupoints sensitization appeared after GMI and disappeared gradually during the natural self-recovery of the gastric mucosa.More SP and CGRP positive nerve fibers were distributed in EB dots than in regions beside EB dots and in the control,mostly distributed in the nerve fibers around both the vessels and root of hair follicle.Mast cells also aggregated and degranulated to release algogenic substances of 5-HT and HA around the vesse

关 键 词：穴位 局部 穴 本态 组织化学 

分 类 号：R245.2[医药卫生—针灸推拿学]