2009-2011年上海ARTI人群中小RNA病毒感染的分子流行病学研究  

Epidemiology and clinical characteristics of patients from Shanghai who had acute respiratory tract infections with picornavirus from 2009-2011

作　　者：何静[1] 徐磊[1] 王蔚[1] 管文彩[1] 田棣[1] 张万菊[1] 刘祎[1] 钱方兴[3] 揭志军 俞慧菊[5] 李杨[6] 袁正宏[2] 胡芸文[1] 

机构地区：[1]上海市(复旦大学附属)公共卫生临床中心,201508 [2]复旦大学医学院分子病毒学重点实验室 [3]上海市长宁区中心医院 [4]上海市第五人民医院 [5]上海交通大学医学院附属新华医院 [6]同济大学附属东方医院

出　　处：《中华实验和临床病毒学杂志》2015年第5期413-416,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 探讨上海地区急性呼吸道感染（ARTI）人群中小RNA病毒感染的流行规律及临床特征.方法 采用套式多重RT-PCR方法,同时检测鼻咽拭子标本小RNA病毒和其他8种常见呼吸道病毒.对其中小RNA病毒阳性标本进行基因分型,并进一步分析其流行病学和临床特征.结果 2282份鼻咽拭子标本中检出小RNA病毒阳性185例（8.1％）,其中鼻病毒（HRV）为151（81.6％）例,肠道病毒（EV）为34（18.4％）例.HRV-A（61.6％）是HRV的主要亚型.HRV在0-4岁组及65岁以上组中检出率高于FluA、RSV等常见呼吸道病毒.HRV和EV在不同季节均有检出,其中HRV在秋冬季达到高峰.HRV引起的URTI和LRTI的比例分别为62.3％ （94/151）、37.8％ （57/151）,而EV感染病例均为URTI.HRV感染阳性的LRTI病例中亚型与LRTI的发生无显著相关性（P＞0.05）.结论 本研究表明,2009-2011年小RNA病毒成为上海地区急性呼吸道感染谱的重要组成部分,其中HRV（HRV-A）为主要病原.HRV是0-4岁组及65岁以上组中最常见的呼吸道病毒,并以秋冬两季为高发季节.HRV亚型与临床重症并无相关性.Objective The present paper aims to investigate the epidemiological and clinical characteristics of patients with ARTIs infections in a large cohort.Methods Nasopharyngeal swabs of patients with ARTIs from Shanghai during May 2009 to April 2011.The nested multiplex reverse transcriptase-polymerase chain reaction （RT-PCR） was used to detect picornavirus and other 8 respiratory viruses.All picornavirus samples were subtyped by nested RT-PCR that targeted to VP4/2 or VP1 region.Results Among the 2282 nasopharyngeal swabs,the detection rate of picornavirus was 8.1% and they were consisted with HRV （151,81.6%） and EV （34,18.4%）.HRV-A （93/151,61.6%） was the most common species of HRV.HRV and RSV were most common in patients younger than 5 years-old,and HRV and Flu A were most common in patients who were at least 65 years-old.HRV-A was the most common HRV species of all age groups.A total of 57 HRV-infected patients （37.8%） presented with lower respiratory tract infections （LRTIs）,including HRV-A （61.6%）,HRV-B （14.1%） and HRV-C （24.5%）.There was no correlation between the HRV species and LRTI.Conclusions Our study indicated that picornavirus was the important pathogen in ARTI patients in Shanghai from May 2009 to April 2011,espacially HRV （HRV-A）.HRV was the most common virus in the younger than 5 years-old and the at least 65 years-old group,the peak season was in autum/winter.No specific HRV subtype correlated with LRTI.

关 键 词：呼吸道感染 RNA 病毒 

分 类 号：R373[医药卫生—病原生物学]