草菇耐低温突变菌株Vtlt-1和原始菌株V23转录组差异的研究  

Differential transcriptome analysis between a mutant strain with low-temperature tolerance and its parental strain

作　　者：吕贝贝[1,2] 于海龙[3] 祝子坪[4] 蒋玮[1,2] 何建华[1,2] 吴潇[1,2] 李鹏[1,2] 王金斌[1,2] 武国干[1,2] 谭琦[4] 唐雪明[1,2] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海市农业科学院食用菌研究所,上海201106 [4]台州学院生命科学学院,浙江台州318000

出　　处：《菌物学报》2015年第6期1143-1152,共10页Mycosystema

基　　金：国家博士后基金(2013M531197);上海市博士后基金(13R21421000);上海市市级农口系统青年人才成长计划[沪农青字(2014)第1-15号];上海市农委科技兴农项目[沪农科攻字2015第(5-6)号]~~

摘　　要：为了培育草菇耐低温菌株与解析其耐低温的分子机制,采用紫外诱变的方法,选育出耐低温草菇菌株Vtlt-1,并利用表达谱芯片技术,比较突变菌株Vtlt-1与原始菌株V23的表达差异基因,筛选差异显著基因后利用GO(gene ontology)功能注释和KEGG(kyoto encyclopedia of genes and genomes)通路富集等方法分析,结果发现与V23相比Vtlt-1表达显著差异基因共有1 600个,其中704个基因上调表达,896个基因下调表达。针对差异基因的GO功能分类结果发现:生物学过程方面,差异基因主要分布在金属离子结合、氧化还原过程、碳水化合物的代谢与核酸的结合。细胞组分方面主要与细胞核相关;分子功能中,氧化还原活性以及解旋酶活性,依赖于ATP的解旋酶活性、DNA指导的RNA聚合酶活性。KEGG注释结果发现差异表达基因主要富集在氨基酸与氮类物质的代谢、脂肪酸与生物碱的合成这两方面,此外还富集到核糖体的生物合成、细胞色素P450、RNA聚合酶、硫和氨基酸的代谢、核酸的修复等通路。这些结果为解析草菇耐低温机制提供分子依据和理论基础。This work was designed to obtain the mutant strain of Volvariello volvacea with low-temperature tolerance and analyze the differentially expressed genes between the mutant and parental strain. By UV mutagenesis, a mutant strain Vtlt-2 was obtained with low-temperature tolerance. Using cDNA microarray, differently expressed genes between Vtlt-1 and parental strain V23 were selected and analysed by GO （Gene ontology） and KEGG （Kyoto encyclopedia of genes and genomes）. There were 2 600 significant differently expressed genes in all, among which 704 genes were up-regulated and 896 genes down-regulated. These differently expressed genes were classified accord biological process were enriched in involvement of metal ion binding, ox ng ida to the GO database. The genes concerned in tion-reducing process, carbohydrate metabolic process and nucleic acid binding, while those concerned in cellular component were related to nucleus. The genes with molecular function were enriched in oxidoreductase activity, helicase activity, ATP-dependent helicase activity and DNA-directed RNA polymerase activity. To determine the function and relationship of these differentially expressed genes, molecular pathway analysis based on the KEGG database were performed. These genes were enriched in amino acid and nitrogen metabolism, fatty acid and alkaloid biosynthesis, ribosome biogenesis in eukaryotes, cytochrome P450, sulfur and amino acid metabolism and nucleotide repair. These results provided a molecular basis for future analysis of the mechanism of low-temperature tolerance in V. volvacea.

关 键 词：草菇 表达谱芯片 耐低温 KEGG代谢通路分析 

分 类 号：S646.13[农业科学—蔬菜学]