马急性滑膜炎模型关节液中MMP-2和MMP-9质量浓度变化的研究  

Changes in synovial fluid MMP-2 and MMP-9 from horses with experimentally-induced synovitis

作　　者：范晓静[1] 王冠颖[1] 姜仁礼 李欣然[1] 李玥[1] 刘旭[1] 王晶璐 潘佳亮[1] 陈希[1] 郑宇[1] 陈蕊[1] 曹馨方[1] 马云峰[1] 赵洋[1] 高利[1] 

机构地区：[1]东北农业大学动物医学学院外科教研室,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2015年第10期1097-1100,共4页Chinese Veterinary Science

基　　金：国家科技支撑计划项目(2012BAD46B02-03)

摘　　要：为探索基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)在马急性滑膜炎模型中的作用,选用13匹蒙古马,将其随机分为试验组(n=8)和对照组(n=5)。在试验组马右侧腕关节内注入0.5ng/mL大肠杆菌脂多糖1mL,建立马滑膜炎模型;在对照组马右侧腕关节注射1mL磷酸盐缓冲液。注射后第0、8、24和168小时各采集关节液1次,用ELISA检测马关节液中MMP-2和MMP-9的质量浓度。结果显示,试验组马在注射后第8小时关节液中MMP-2、MMP-9质量浓度显著增加并达到最高,分别为18.05ng/mL±0.49ng/mL和19.64ng/mL±0.59ng/mL,与对照组比较差异显著。注射后第24小时MMP-2、MMP-9质量浓度下降,第168小时质量浓度恢复到对照组水平。结果表明,MMP-2和MMP-9在滑膜炎的急性期起到重要作用;MMP-2和MMP-9可以作为检测马滑膜炎的指标。The study aimed to explore the effects of matrix metalloproteinase-2（MMP-2）and matrix metalloproteinase-9（MMP-9）in acute synovitis of the horse.Thirteen horses were divided into experimental（n=8）and control groups（n=5）,acute synovitis was induced in 8horses by 1mL Escherichia coli lipopolysaccharide（0.5ng/mL）into the right middle carpal joint,the control group was injected into the right middle carpal joint of 1mL phosphate buffer saline.Synovial fluid was collected at hour post-injection（hpi）0,8,24 and 168.Concentrations of MMP-2and MMP-9were measured with ELISA kit.In result,induction of acute synovitis led to a sharp rise in MMP-2and MMP-9and returned to peaking at hpi 8,18.05ng/mL±0.49ng/mL and 19.64ng/mL±0.59ng/mL,then to decline at hpi 24 and returned to baseline at hpi 168,the differences were significantly compared with control group.The results indicated MMP-2and MMP-9plays important role in the early acute developmental phase of equine synovitis,thus can be indicators of monitoring acute synovitis.

关 键 词：急性滑膜炎 脂多糖 基质金属蛋白酶-2 基质金属蛋白酶-9 马 

分 类 号：S857.165[农业科学—临床兽医学]