纳米银参与的化学发光法测定蜘蛛香中的香叶木素  

Determination of diosmetin in Valeriana jatamansi Jones by chemiluminescence method participated by nano silver

作　　者：李丽[1] 周红暇 刘焱[1] 王科玲 左学红 王灿[1] 

机构地区：[1]遵义师范学院化学化工学院黔北特色资源应用研究实验室,贵州遵义563002

出　　处：《中国卫生检验杂志》2015年第20期3436-3438,3444,共4页Chinese Journal of Health Laboratory Technology

基　　金：贵州省2014年大学生创新创业训练计划项目(20141-0664003);贵州省科学技术基金项目(项目合同编号:黔科合J字LKZS[2012]16号);遵义师范学院2014-2015学年度大学生科研训练计划项目(理科类课题;14);遵义师范学院2014-2015学年度大学生科研训练计划项目(理科类课题;31)

摘　　要：目的建立化学发光法测定蜘蛛香中香叶木素的新方法。方法在纳米银参与的碱性介质中,高锰酸钾-鲁米诺-香叶木素体系产生较强的化学发光反应且发光强度与香叶木素的浓度有很好的线性关系,据此建立流动注射化学发光法测定香叶木素的含量。结果在鲁米诺、Na OH、纳米银、KMn O4溶液浓度分别为8.0×10-7mol/L、0.05 mol/L、5.0×10-6mol/L、3.0×10-5mol/L的最佳条件下,香叶木素的线性范围为5.0×10-9g/ml^5.0×10-7g/ml,r=0.998 7,对1.0×10-7g/ml香叶木素平行测定11次,其相对标准偏差RSD为1.88%,检出限为1.8×10-9g/ml,提出了化学发光反应可能的机理。结论该法简单、准确,可用于蜘蛛香中香叶木素的测定。Objective To establish a new method for determination of diosmetin in Valeriana jatamansi Jones by chemiluminescent system. Methods In the alkaline medium participated by nano silver,the chemiluminescent intensity of KMn O4-luminol-diosmetin system had a good linear relationship with diosmetin. Based on this,a new method of flow injection chemiluminescent system for detection of diosmetin was developed. Results Under the best conditions of luminol,Na OH,nano silver and KMn O4,whose solution concentration were respectively 8. 0 × 10^-7mol / L,0. 05 mol / L,5. 0 × 10^-6mol / L and 3. 0 ×10-5mol / L,the linear range of diosmetin were 5. 0 × 10^-9g / ml-5. 0 × 10^-7g / ml,r = 0. 998 7. Parallel determination of 1 ×10^-7g / ml diosmetin were conducted for 11 times,whose relative standard deviation was 1. 88% and the detection limit was1. 8 × 10^-9g / ml. The possible mechanism of this chemiluminescence reaction was also proposed. Conclusion The method is simple,accurate and it can be used for determination of diosmetin in Valeria jatamansi Jones.

关 键 词：纳米银 化学发光 香叶木素 鲁米诺 

分 类 号：R284.1[医药卫生—中药学]