HPLC法测定灵丹多糖胶囊中丹酚酸B的含量  

Determination of Salvianolic Acid B in Lingdanduotang Capsules by HPLC

作　　者：翁立冬[1] 乡世健[1] 洪军辉 曹思玮[1] 朱红霞[1] 吕志平[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《今日药学》2015年第10期712-714,共3页Pharmacy Today

摘　　要：目的建立灵丹多糖胶囊中丹酚酸B含量的高效液相色谱法分析方法。方法采用Agilent HC-C18（4.6 mm×250 mm,5μm）为色谱柱;0.2%磷酸溶液-乙腈（77∶23）为流动相;流速为1.0 m L/min;检测波长为286 nm;柱温为25℃。结果丹酚酸B浓度在0.556-2.78μg/m L范围内呈现良好的线性关系,平均加样回收率为99.34%,RSD为0.58%（n=6）。结论本法操作简便、结果准确、重复性良好,可作为灵丹多糖胶囊的含量控制方法。OBJECTIVE To establish a determination method for Salvianolic acid B in Lingdanduotang capsules by HPLC.METHODS The Agilent HC-C18（ 4.6 mm x 250 mm,5 μm） was used as the column; 0.1% phosphoric acid solution-acetonitrile（ 77 ∶23） was used as the mobile phase; the flow rate was 1.0 m L / min; the detection wavelength was 286 nm; and the temperature was 25℃.RESULTS The sample volume of Salvianolic acid B was linear in the range of 0. 556- 2. 78 μg / m L,the average recovery was99. 34% and RSD was 0. 58%（ n = 6）. CONCLUSION This method is simple,quick and accurate,and it can be used for the determination of Salvianolic acid B in Lingdanduotang capsules.

关 键 词：灵丹多糖胶囊 丹酚酸B HPLC 含量测定 

分 类 号：R284[医药卫生—中药学]