基于SERS的大鼠脑组织免疫分析  

Immunoassay of Rat Brain Tissue by Surface-enhanced Raman Spectroscopy

作　　者：杨津[1,2] 刘卓[3] 苏虹羊 彭茹[2] 宋薇[1] 赵冰[1] 

机构地区：[1]吉林大学超分子结构与材料国家重点实验室,长春130012 [2]吉林大学基础医学院,长春130021 [3]吉林大学中日联谊医院血管外科,长春130021

出　　处：《高等学校化学学报》2015年第11期2317-2321,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21273091;21221063;21327803;21411140235);吉林省科技厅青年基金(批准号:20130522181JH;20130522137JH)资助~~

摘　　要：将常规免疫组织化学法与表面增强拉曼散射光谱(SERS)结合,建立了一种简便、快速测定大鼠脑组织中神经元特异性骨架蛋白的新方法.将所制备的SERS免疫胶体金标签加入至大鼠脑组织冰冻切片,研究了经免疫识别后组织的SERS光谱,在脑组织切片中得到了探针分子对巯基苯甲酸(MBA)的特征峰(1076,1589 cm-1).对比了不同浓度抗体对SERS信号及免疫组化染色的影响,结果表明,与传统的免疫组化法相比,SERS法可以简化步骤,缩短实验时间,同时展现出更高的检测灵敏度和选择性.Surface-enhanced Raman scattering（SERS） was used to develop and optimize a novel, quick and simple immunohistochemical ABC （avidin-biotin-HRP complex） assay for detecting specific skeleton protein neurofilament-200 （NF-200） in the frozen sections of rat brain tissue. In this study, anti mouse NF-200 la- beled colloid gold was used as immunoprobe, which was added into the frozen sections of rat brain tissue con- taining NF-200 protein. Intense SERS signals ofp-mercaptobenzoic acid（MBA） at 1076 and 1589 cm-1 were observed clearly after the specific binding of antigen and antibody. Meanwhile, the SERS spectra and the im- munohistochemical staining effect of antigen and antibody complex with different concentrations of anti NF-200 were examined. Compared with the traditional method, this assay can simplify the procedure, moreover, it showed higher sensitivity and selectivity.

关 键 词：神经丝蛋白200 表面增强拉曼光谱 免疫组织化学法 

分 类 号：O657.37[理学—分析化学]