间接酶联免疫吸附试验与传统血清学方法应用于人布鲁杆菌抗体的试验比较  

A comparison between the rapid enzyme-linked immunosorbent assay and the traditional serological methods in diagnosis of human brucellosis

作　　者：何晶晶[1] 赵冬梅[1] 周珣[1] 刘景瑶[1] 郑遵荣[1] 周玉珍[1] 张雁[1] 

机构地区：[1]黑龙江省农垦总局总医院检验科,哈尔滨150088

出　　处：《中华地方病学杂志》2015年第11期851-853,共3页Chinese Journal of Endemiology

摘　　要：目的探讨间接酶联免疫吸附试验（IELISA）与传统血清学方法在人布鲁杆菌抗体的检测中的差别。方法采用IELISA、虎红平板凝集试验（RBPT）、试管凝集试验（SAT）、抗人免疫球蛋白试验（Coomb’s试验）和半胱氨酸试验方法对430例确诊及疑似布病患者（病例组）和同期300例健康体检者（健康组）进行布鲁杆菌抗体检测。分析IEUSA方法与RBPT、SAT、Coomb’S试验、半胱氨酸试验的符合程度。结果病例组IELISA、RBPT、SAT、Coomb’8试验及半胱氨酸试验的布鲁杆菌抗体检测阳性率高于健康组（IELISA：83．49％比0；RBPT：86．97％比0；SAT：76．27％比0；Coomb’s试验：65．58％比0．02％；半胱氨酸试验：67．91％比0，χ2=535．05、412．47、437．66、339．22、489．49，P均〈0．01）。IELISA与RBPT、SAT、Coomb’s试验、半胱氨酸试验检测布鲁杆菌抗体阳性符合率分别为87．19％、79.39％、71．59％和76．60％，其中IELISA与RBPT的阳性符合率最高，而与Coomb’s试验的阳性符合率最低。结论RBPT和IELISA的联合应用可简便、快速、准确的检测布鲁杆菌抗体，为布鲁杆菌病的防治提供重要技术支撑，值得推广应用。Objective To investigate the differents of rapid enzyme-linked immunosorbent assay（IELISA） and the traditional serological methods in diagnosis of human brucellosis. Methods BruceUa antibody was detected by IELISA, rose bangel plate test （RBPT）, standard agglutination test （SAT）, Coomb＇s test and cysteine test of serological methods in 430 confirmed and suspected patients and 300 healthy controls during the same period. The consistency was analyzed between IELISA and other tests. Results Positive rates of patients were significantly higher than those in healthy controls （IELISA： 83.49% vs. 0; RBPT： 86.97% vs. 0; SAT： 76.27% vs. 0; Coomb＇s： 65.58% vs. 0.02%; and cysteine test： 67.91% vs. 0, χ2 = 535.05, 412.47, 437.66, 339.22, 489.49, all P 〈 0.01）. Positive consistency rates between IELISA and other tests were 87.19%, 79.39%, 71.59% and 76.60%, the highest was between IELISA and RBPT, the lowest was between IELISA and Goomb＇s, Conclusions Brttcella antibody is detected conveniently, quickly, and accurately by the joint application of RBPT and IELISA, which provides important technical support for prevention and control of brucellosis. It is worthy of application extensively.

关 键 词：间接酶联免疫吸附试验 虎红平板凝集试验 试管凝集试验 抗人免疫球蛋白试验 布鲁杆菌病 

分 类 号：R516.7[医药卫生—内科学] R446.6[医药卫生—临床医学]