硫化氢对大鼠急性脊髓损伤的保护作用  

Protective effects of hydrogen sulfide on acute spinal cord injury in rats

作　　者：孙平[1] 范仲凯[1] 李昊天[1] 王继权[1] 赵兴长 李刚[1] 吕刚[1] 

机构地区：[1]辽宁医学院附属第一医院骨科,121001

出　　处：《天津医药》2015年第11期1271-1274,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81272074);辽宁省高等学校优秀人才支持计划项目(LJQ2014091);辽宁医学院校长基金(QM2014010)

摘　　要：目的探讨硫化氢(H2S)对大鼠急性脊髓损伤后自噬及细胞凋亡的影响。方法 36只健康雄性SD大鼠随机分为假手术组(Sham组)、单纯脊髓损伤组(模型组)、H2S处理组(H2S组),每组12只。采用Allen’s法构建大鼠脊髓损伤模型,Sham组只行椎板切除术,暴露脊髓不打击;H2S组在脊髓损伤后1 h腹腔注射50μmol/kg硫氢化钠;模型组给予等体积生理盐水。各组脊髓损伤24 h后取出脊髓组织,Western blot检测LC3、p70S6K和Cleaved cas-pase-3表达;免疫荧光法检测LC3表达;TUNEL染色法检测各组细胞凋亡率。结果与Sham组相比,模型组LC3Ⅱ/LC3Ⅰ,Cleaved caspase-3表达升高,p70S6K表达降低、细胞凋亡率升高(P<0.01);与模型组相比,H2S组LC3Ⅱ/LC3Ⅰ、Cleaved caspase-3表达降低,p70S6K表达升高,细胞凋亡率下降(P<0.01)。结论 H2S可抑制大鼠急性脊髓损伤后自噬并减少细胞凋亡。Objective To investigate the effects of hydrogen sulfide on autophagy and the apoptosis after acute spinal cord injury in rats. Methods Thirty-six adult male SD rats （250-300 g） were randomly divided into three groups （n=12 for each group）：sham operation group （Sham group）, spinal cord injury group （Model group） and hydrogen sulfide pre-treatment group （H2S group）. Allen’s method was used to establish the rat model of spinal cord injury. Rats of sham operation group re-ceived only laminectomy. Rats of H2S group received sodium hydrosulphide injection intraperitoneally （50μmol/kg） 1h after spinal cord injury, and Model group was given the same amount of saline solution. Rats in the three groups were sacrificed 24 h after spinal cord injury, then the spinal cord was removed. The expressions of LC3, p70S6K and Cleaved caspase-3 were detected by Western blot assay. The expression of LC3 was also detected by immunofluorescence. The cell apoptosis was as-sessed by TUNEL stain. Results Compared with Sham group, the expression levels of LC3Ⅱ/LC3Ⅰand Cleaved caspase-3 were increased in Model group, but the expression of p70S6K decreased and cell apoptosis increased in Model group （P〈0.01）. Compared with Model group, the expression levels of LC3Ⅱ/LC3Ⅰand Cleaved caspase-3 were decreased significant-ly, while the expression of p70S6K increased and cell apoptosis decreased significantly in H2S group （P 〈 0.01）. Conclu-sion Hydrogen sulfide can inhibit autophagy and reduce cell apoptosis after acute spinal cord injury in rats.

关 键 词：急性脊髓损伤 硫化氢 自噬 LC3 细胞凋亡 印迹法 蛋白质 

分 类 号：R651.21[医药卫生—外科学]